利用抑制性消减杂交衍生的特异性引物定量环境样品中的丙酸丙酸杆菌 P169 细菌。
Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific primers derived by suppressive subtractive hybridization.
机构信息
Danisco Cultures Division, W227 N752 Westmound Drive, Waukesha, WI 53186, USA.
出版信息
Appl Environ Microbiol. 2011 Jun;77(11):3898-902. doi: 10.1128/AEM.02586-10. Epub 2011 Apr 1.
Abstract
A quantitative PCR (qPCR) assay targeting a gene identified by suppressive subtractive hybridization (SSH) was developed to detect Propionibacterium acidipropionici P169, with a threshold of 10(4) CFU/U of dairy feed or rumen fluid. The report is the first using a molecular marker generated by SSH to quantify a bacterial strain in environmental samples.
摘要
建立了一种针对抑制性消减杂交（SSH）鉴定基因的定量 PCR（qPCR）检测方法，用于检测丙酸丙酸杆菌 P169，乳制品饲料或瘤胃液的检测限为 10（4）CFU/U。这是首次使用 SSH 产生的分子标记定量环境样品中的细菌菌株。
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