Costa E, Teixidó N, Usall J, Atarés E, Viñas I
Postharvest Unit, CeRTA, Centre UdL-IRTA, Lleida, Spain.
Appl Microbiol Biotechnol. 2001 Aug;56(3-4):367-71. doi: 10.1007/s002530100666.
The aim of this paper was to find the nitrogen and carbon sources that provide maximum biomass production of strain CPA-2 of the biocontrol agent Pantoea agglomerans and minimum cost of media, whilst maintaining biocontrol efficacy. To reduce the cost of media, commercial products and by-products were tested. P. agglomerans can be produced using a combination of nitrogen sources such as yeast extract (5 g l(-1)) and dry beer yeast (10 g l(-1)) with inexpensive carbohydrates such as sucrose (10 g l(-1)) and molasses (20 g l(-1)), respectively, maintaining the efficacy of the biocontrol agent against Penicillium digitatum and P. italicum on oranges. The results obtained in this study could be used to provide a reliable basis for a scale-up of this fermentation process to an industrial level.
本文的目的是找到能使生物防治剂成团泛菌CPA-2产生最大生物量且培养基成本最低的氮源和碳源，同时保持生物防治效果。为降低培养基成本，对商业产品和副产品进行了测试。成团泛菌可以分别使用酵母提取物（5 g l(-1)）和干啤酒酵母（10 g l(-1)）等氮源与蔗糖（10 g l(-1)）和糖蜜（20 g l(-1)）等廉价碳水化合物的组合来生产，同时保持该生物防治剂对橙子上指状青霉和意大利青霉的防治效果。本研究获得的结果可为将该发酵工艺扩大到工业规模提供可靠依据。
