Fernandes Paulo, Afonso Isabel Maria, Pereira Jéssica, Rocha Rui, Rodrigues Ana Sofia
CISAS, Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Álvares, 4900-347 Viana do Castelo, Portugal.
CISAS, Escola Superior Agrária, Instituto Politécnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Álvares, 4900-347 Viana do Castelo, Portugal.
Biology (Basel). 2023 Jan 18;12(2):146. doi: 10.3390/biology12020146.
Geographic location and, particularly, soil and climate exert influence on the typicality of a wine from a specific region, which is often justified by the , and these factors also influence the epiphytic flora associated with the surface of the grape berries. In the present study, the microbiome associated with the surface of berries obtained from ten vineyards of the variety located in different geographical locations in mainland Portugal was determined and analyzed. The removal of microbial flora from the surface of the berries was carried out by washing and sonication, after which the extraction and purification of the respective DNA was carried out. High-throughput short amplicon sequencing of the fungal ITS region and the bacterial 16S region was performed, allowing for the determination of the microbial consortium associated with wine grapes. Analysis of α-diversity demonstrated that parcels from the Monção and Melgaço sub-region present a significantly ( < 0.05) lower fungal diversity and species richness when compared to the plots analyzed from other regions/sub-regions. The ubiquitous presence of spp., a yeast with enologic potential interest in all parcels from Monção and Melgaço, was also observed.
地理位置，尤其是土壤和气候，会对特定地区葡萄酒的典型性产生影响，这通常是合理的，而且这些因素也会影响与葡萄浆果表面相关的附生植物群。在本研究中，对从葡萄牙大陆不同地理位置的十个 品种葡萄园收获的浆果表面相关的微生物群进行了测定和分析。通过洗涤和超声处理去除浆果表面的微生物菌群，之后进行各自DNA的提取和纯化。对真菌ITS区域和细菌16S区域进行了高通量短扩增子测序，从而确定与酿酒葡萄相关的微生物群落。α多样性分析表明，与其他地区/次区域分析的地块相比，蒙桑和梅尔加索次区域的地块真菌多样性和物种丰富度显著更低（<0.05）。还观察到在蒙桑和梅尔加索所有地块中均普遍存在具有酿酒潜在价值的酵母 属物种。
