Das Nabajit, Tripathi Naveen, Basu Srijoni, Bose Chandra, Maitra Susmit, Khurana Sukant
Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata Kolkata, India.
School of Biotechnology, Kalinga Institute of Industrial Technology University Bhubaneswar, India.
Front Microbiol. 2015 Jul 23;6:698. doi: 10.3389/fmicb.2015.00698. eCollection 2015.
Gelling agents are required for formulating both solid and semisolid media, vital for the isolation of microorganisms. Gelatin was the first gelling agent to be discovered but it soon paved the way for agar, which has far superior material qualities. Source depletion, issues with polymerase-chain-reaction and inability to sustain extermophiles etc., necessitate the need of other gelling agents. Many new gelling agents, such as xantham gum, gellan gum, carrageenan, isubgol, and guar gum have been formulated, raising the hopes for the growth of previously unculturable microorganisms. We evaluate the progress in the development of gelling agents, with the hope that our synthesis would help accelerate research in the field.
配制固体和半固体培养基都需要凝胶剂,这对于微生物的分离至关重要。明胶是最早被发现的凝胶剂,但它很快就为琼脂铺平了道路,琼脂具有更优越的材料特性。资源枯竭、聚合酶链反应问题以及无法维持嗜极端微生物等情况,使得需要其他凝胶剂。许多新型凝胶剂,如黄原胶、结冷胶、卡拉胶、车前子壳粉和瓜尔豆胶等已被配制出来,为以前无法培养的微生物的生长带来了希望。我们评估了凝胶剂开发方面的进展,希望我们的综述将有助于加速该领域的研究。