Cao Qinhong, Chen Jie, Zhu Li, Liu Yun, Zhou Zuomin, Sha Jiahao, Wang Shui, Li Jianmin
Lab of Reproductive Medicine, Department of Cell Biology and Medical Genetics, Nanjing Medical University, Han Zhong Road 140, Nanjing, Jiangsu Province, China.
Biochem Biophys Res Commun. 2006 Jun 9;344(3):798-806. doi: 10.1016/j.bbrc.2006.03.208. Epub 2006 Apr 19.
Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast cancer. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. To date, three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. Herein, we identified a novel TPD52-like sequence (TPD52L3, or hD55) in human testis using cDNA microarray. Sequence analysis of the deduced protein suggests that hD55 contains a coiled-coil motif and is highly conserved compared with other TPD52-like sequences. Yeast two-hybrid and GST pull-down assays revealed that hD55 interacts with hD52, hD53, hD54, and itself. cDNA microarray detection found that hD55 was expressed at 5.6-fold higher levels in adult testis than in fetal testis. Additionally, the expression profile shows that hD55 is testis-specific, indicating a potential role for hD55 in testis development and spermatogenesis.
肿瘤蛋白D52样蛋白（TPD52）是一类带有小卷曲螺旋基序的蛋白质，最初在乳腺癌中被发现。TPD52及相关蛋白与细胞增殖、凋亡和囊泡运输有关。迄今为止，已鉴定出三个人类TPD52成员，分别命名为hD52（TPD52）、hD53（TPD52L1）和hD54（TPD52L2）。该蛋白家族最重要的特征是一个高度保守的卷曲螺旋基序，它是与其他TPD52样蛋白进行同源和异源相互作用所必需的。在此，我们利用cDNA微阵列在人类睾丸中鉴定出一个新的TPD52样序列（TPD52L3，或hD55）。对推导蛋白的序列分析表明，hD55含有一个卷曲螺旋基序，与其他TPD52样序列相比高度保守。酵母双杂交和GST下拉实验表明，hD55与hD52、hD53、hD54以及自身相互作用。cDNA微阵列检测发现，hD55在成人睾丸中的表达水平比胎儿睾丸高5.6倍。此外，表达谱显示hD55是睾丸特异性的，表明hD55在睾丸发育和精子发生中具有潜在作用。
