Schuldiner M, Eiges R, Eden A, Yanuka O, Itskovitz-Eldor J, Goldstein R S, Benvenisty N
Department of Genetics, The Institute of Life Sciences, The Hebrew University, Givat-Ram Campus, 91904, Jerusalem, Israel.
Brain Res. 2001 Sep 21;913(2):201-5. doi: 10.1016/s0006-8993(01)02776-7.
Human embryonic stem (ES) cells are pluripotent cells capable of forming differentiated embryoid bodies (EBs) in culture. We examined the ability of growth factors under controlled conditions to increase the number of human ES cell-derived neurons. Retinoic acid (RA) and nerve growth factor (betaNGF) were found to be potent enhancers of neuronal differentiation, eliciting extensive outgrowth of processes and the expression of neuron-specific molecules. Our findings show that human ES cells have great potential to become an unlimited cell source for neurons in culture. These cells may then be used in transplantation therapies for neural pathologies.
人类胚胎干细胞（ES细胞）是多能细胞，在培养中能够形成分化的胚状体（EBs）。我们在可控条件下检测了生长因子增加人ES细胞来源神经元数量的能力。发现视黄酸（RA）和神经生长因子（βNGF）是神经元分化的有效增强剂，可引发广泛的突起生长和神经元特异性分子的表达。我们的研究结果表明，人ES细胞有很大潜力成为培养中神经元的无限细胞来源。这些细胞随后可用于神经病理学的移植治疗。
