Soyano A, Chinea M, Romano E L
Immunopharmacology. 1984 Dec;8(3-4):163-9. doi: 10.1016/0162-3109(84)90021-3.
This paper reports experiments designed to evaluate the effect of desferrioxamine on lymphocytes obtained from the spleen of Sprague-Dawley rats. The results showed that desferrioxamine inhibits the proliferative response of lymphocytes stimulated by concanavalin A or pokeweed mitogen, the effect being proportional to the dose used, and being maximal at the end of the culture period. To exert its effect, desferrioxamine has to be present from the initiation of the cultures--its addition 24 h later markedly reduced its inhibitory effect. Studies of cell viability indicated that the inhibition was not due to toxicity. Lymphocytes obtained from young animals (4 to 12-week-old) were more sensitive to desferrioxamine than older animals (13 to 18-week-old). Saturation of desferrioxamine with iron abolished its effect, suggesting a direct relationship between chelating properties and inhibition, and reinforcing the hypothesis that iron plays an important role in the process of cell division.
本文报道了旨在评估去铁胺对从斯普拉格-道利大鼠脾脏获取的淋巴细胞作用的实验。结果表明，去铁胺抑制由刀豆球蛋白A或商陆有丝分裂原刺激的淋巴细胞增殖反应，其作用与所用剂量成正比，且在培养期末达到最大。为发挥其作用，去铁胺必须在培养开始时就存在——培养24小时后添加会显著降低其抑制作用。细胞活力研究表明，这种抑制并非由毒性引起。从幼龄动物（4至12周龄）获取的淋巴细胞比老龄动物（13至18周龄）对去铁胺更敏感。用铁使去铁胺饱和会消除其作用，这表明螯合特性与抑制之间存在直接关系，并强化了铁在细胞分裂过程中起重要作用的假说。
