Ren N, Timko M P
Department of Biology, University of Virginia, Charlottesville 22903, USA.
Genome. 2001 Aug;44(4):559-71.
Amplified fragment length polymorphism (AFLP) analysis was used to determine the degree of intra- and inter-specific genetic variation in the genus Nicotiana. Forty-six lines of cultivated tobacco (Nicotiana tabacum L.) and seven wild Nicotiana species, including N. sylvestris, N. tomentosiformis, N. otophora, N. glutinosa, N. suaveolens, N. rustica, and N. longiflora, were analyzed, using at least eight different oligonucleotide primer combinations capable of detecting a minimum of 50 polymorphic bands per primer pair. The amount of genetic polymorphism present among cultivated tobacco lines (N. tabacum) was limited, as evidenced by the high degree of similarity in the AFLP profiles of cultivars collected worldwide. Six major clusters were found within cultivated tobacco that were primarily based upon geographic origin and manufacturing quality traits. A greater amount of genetic polymorphism exists among wild species of Nicotiana than among cultivated forms. Pairwise comparisons of the AFLP profiles of wild and cultivated Nicotiana species show that polymorphic bands present in N. tabacum can be found in at least one of three proposed wild progenitor species (i.e., N. sylvestris, N. tomentosiformis, and N. otophora). This observation provides additional support for these species contributing to the origin of N. tabacum.
扩增片段长度多态性（AFLP）分析用于确定烟草属内种内和种间的遗传变异程度。分析了46个栽培烟草（Nicotiana tabacum L.）品系和7个野生烟草物种，包括林烟草（N. sylvestris）、绒毛状烟草（N. tomentosiformis）、耳状烟草（N. otophora）、粘毛烟草（N. glutinosa）、香烟草（N. suaveolens）、黄花烟草（N. rustica）和长花烟草（N. longiflora），使用了至少8种不同的寡核苷酸引物组合，每个引物对能够检测到至少50条多态性条带。栽培烟草品系（N. tabacum）之间存在的遗传多态性数量有限，这从全球收集的栽培品种的AFLP图谱的高度相似性可以看出。在栽培烟草中发现了6个主要聚类，主要基于地理起源和加工品质性状。烟草属野生物种中的遗传多态性比栽培类型中的更多。野生和栽培烟草物种的AFLP图谱的成对比较表明，烟草中存在的多态性条带可以在三个假定的野生祖先物种（即林烟草、绒毛状烟草和耳状烟草）中的至少一个中找到。这一观察结果为这些物种对烟草起源的贡献提供了额外的支持。
