Julio E, Verrier J-L, Dorlhac de Borne F
Altadis, Institut du Tabac, Domaine de la Tour, LBCM, 24100 Bergerac, France.
Theor Appl Genet. 2006 Jan;112(2):335-46. doi: 10.1007/s00122-005-0132-y. Epub 2005 Nov 11.
Amplified fragment length polymorphism (AFLP) was conducted on a set of 92 Nicotiana tabacum L. accessions from diverse types (flue-cured, dark air-cured, burley, oriental, and cigar wrapper) and breeding origins to identify markers associated with disease resistances. Eleven primer combinations were required to identify 33 polymorphic fragments. This allowed the identification of 92% of these accessions, and yielded sufficient information for building a neighbor joining tree. Clusters of accessions with common traits or breeding origins were observed. An important part of this polymorphism could be related to interspecific introgressions from other Nicotiana species, performed during the breeding history of N. tabacum to confer resistance to pathogens. Seven fragments were associated with three different resistances: two for the blue-mold (Peronospora tabacina Adam) resistance derived from Nicotiana debneyi Domin, two for the Va gene (Potato Virus Y susceptibility), and three for the black root rot (Chalara elegans) resistance of N. debneyi origin. Some of these markers were converted into sequence characterized amplified region markers, and validated on recombinant inbred lines or doubled-haploid lines.
对92份来自不同类型（烤烟、深色晾烟、白肋烟、香料烟和雪茄包皮烟）和育种起源的烟草（Nicotiana tabacum L.）种质进行了扩增片段长度多态性（AFLP）分析，以鉴定与抗病性相关的标记。需要11对引物组合来鉴定33个多态性片段。这使得能够鉴定出92%的这些种质，并产生了足够的信息来构建邻接树。观察到具有共同性状或育种起源的种质聚类。这种多态性的一个重要部分可能与烟草在育种历史中为获得对病原体的抗性而从其他烟草物种进行的种间渐渗有关。7个片段与三种不同的抗性相关：两个与源自德氏烟草（Nicotiana debneyi Domin）的抗蓝霉病（烟草霜霉病菌Peronospora tabacina Adam）相关，两个与Va基因（马铃薯Y病毒易感性）相关，三个与源自德氏烟草的抗黑根腐病（优美长蠕孢Chalara elegans）相关。其中一些标记被转化为序列特征性扩增区域标记，并在重组自交系或双单倍体系中进行了验证。
