Department of Agronomy, Zhejiang University, Hangzhou 310058, China.
J Zhejiang Univ Sci B. 2013 Jul;14(7):570-7. doi: 10.1631/jzus.B1200227.
Diversity arrays technology (DArT) is a microarray-based marker system that achieves high throughput by reducing the complexity of the genome. A DArT chip has recently been developed for tobacco. In this study, we genotyped 267 flue-cured cultivars/landraces, including 121 Chinese accessions over five decades from widespread geographic regions in China, 103 from the Americas, and 43 other foreign cultivars, using the newly developed chip. Three hundred and thirty polymorphic DArT makers were selected and used for a phylogenetic analysis, which suggested that the 267 accessions could be classified into two subgroups, which could each be further divided into 2‒4 sections. Eight elite cultivars, which account for 83% of the area of Chinese tobacco production, were all found in one subgroup. Two high-quality cultivars, HHDJY and Cuibi1, were grouped together in one section, while six other high-yield cultivars were grouped into another section. The 330 DArT marker clones were sequenced and close to 95% of them are within non-repetitive regions. Finally, the implications of this study for Chinese flue-cured tobacco breeding and production programs were discussed.
多样性数组技术（DArT）是一种基于微阵列的标记系统，通过降低基因组的复杂性实现高通量。最近为烟草开发了一种 DArT 芯片。在这项研究中，我们使用新开发的芯片对来自中国广泛地理区域的 267 个烤烟品种/地方品种进行了基因型分析，其中包括 121 个来自中国的品种，来自美洲的 103 个品种和 43 个其他外国品种。选择了 330 个多态性 DArT 标记用于系统发育分析，该分析表明，267 个品种可分为两个亚组，每个亚组可进一步分为 2-4 个部分。占中国烟草种植面积 83%的 8 个优良品种均位于一个亚组中。两个优质品种 HHDJY 和 Cuibi1 位于同一部分，而其他 6 个高产品种位于另一部分。对 330 个 DArT 标记克隆进行测序，其中近 95%位于非重复区域。最后，讨论了这项研究对中国烤烟育种和生产计划的意义。
