Fedriani J R, Marino A, Macarulla J M, Ugarte J, Rodríguez C
Rev Esp Fisiol. 1981 Mar;37(1):97-102.
A method is described for the extraction and thin-layer chromatographic separation of phospholipids. Eight separate phospholipid fractions are easily distinguishable. Liver phospholipid metabolism in Gallus domesticus has been studied in newborn chicken (less than 24 h old). (1-14C) serine, 3-14C) serine, (2-14C) ethanolamine and (methyl-14C) choline were used as radioactive precursors. Serine is incorporated as lysophosphatidylserine, ethanolamine as phosphatidylethanolamine, and choline as L-alpha-glycerophosphorylcholine. The observed pathways for serine and ethanolamine incorporation and turnover are the same as those described for mammals; the main pathway for choline is the synthesis of phosphatidylcholine from lysophosphatidylcholine.
本文描述了一种磷脂提取及薄层色谱分离的方法。八个独立的磷脂组分易于区分。在家鸡新生雏鸡（小于24小时龄）中研究了肝脏磷脂代谢。使用（1-¹⁴C）丝氨酸、（3-¹⁴C）丝氨酸、（2-¹⁴C）乙醇胺和（甲基-¹⁴C）胆碱作为放射性前体。丝氨酸以溶血磷脂酰丝氨酸形式掺入，乙醇胺以磷脂酰乙醇胺形式掺入，胆碱以L-α-甘油磷酸胆碱形式掺入。观察到的丝氨酸和乙醇胺掺入及周转途径与哺乳动物中描述的相同；胆碱的主要途径是由溶血磷脂酰胆碱合成磷脂酰胆碱。
