关于将赖氨酰 - tRNA 导入有袋类动物线粒体的证据。
Evidence for import of a lysyl-tRNA into marsupial mitochondria.
作者信息
Dörner M, Altmann M, Pääbo S, Mörl M
机构信息
Max-Planck-Institute for Evolutionary Anthropology, D-04105 Leipzig, Germany.
出版信息
Mol Biol Cell. 2001 Sep;12(9):2688-98. doi: 10.1091/mbc.12.9.2688.
The mitochondrial tRNA gene for lysine was analyzed in 11 different marsupial mammals. Whereas its location is conserved when compared with other vertebrate mitochondrial genomes, its primary sequence and inferred secondary structure are highly unusual and variable. For example, eight species lack the expected anticodon. Because the corresponding transcripts are not altered by any RNA-editing mechanism, the lysyl-tRNA gene seems to represent a mitochondrial pseudogene. Purification of marsupial mitochondria and in vitro aminoacylation of isolated tRNAs with lysine, followed by analysis of aminoacylated tRNAs, show that a nuclear-encoded tRNA(Lys) is associated with marsupial mitochondria. We conclude that a functional tRNA(Lys) encoded in the nuclear genome is imported into mitochondria in marsupials. Thus, tRNA import is not restricted to plant, yeast, and protozoan mitochondria but also occurs also in mammals.
对11种不同的有袋类哺乳动物的线粒体赖氨酸tRNA基因进行了分析。与其他脊椎动物线粒体基因组相比，其位置是保守的，但其一级序列和推断的二级结构却非常独特且多变。例如，有8个物种缺少预期的反密码子。由于相应的转录本不会被任何RNA编辑机制改变，赖氨酸tRNA基因似乎代表一个线粒体假基因。对有袋类动物线粒体进行纯化，并将分离出的tRNA用赖氨酸进行体外氨酰化，随后对氨酰化的tRNA进行分析，结果表明，一种核编码的tRNA（Lys）与有袋类动物线粒体相关。我们得出结论，有袋类动物中，核基因组编码的功能性tRNA（Lys）被导入线粒体。因此，tRNA的导入并不局限于植物、酵母和原生动物的线粒体，在哺乳动物中也会发生。
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