Janke A, Pääbo S
Institute of Zoology, University of Munich, Germany.
Nucleic Acids Res. 1993 Apr 11;21(7):1523-5. doi: 10.1093/nar/21.7.1523.
RNA editing has been described for organellar genes of protozoa and plants as well as in three cases for nuclear transcripts of mammals. The mitochondrial tRNA for aspartic acid is encoded in the mitochondrial genome of marsupials with the anticodon GCC. However, cDNA from the gene product revealed that the second nucleotide of the anticodon of the tRNA is posttranscriptionally changed to be recognized as an uridine residue. This restores the coding property of the tRNA. Direct sequencing as well as clones of the amplified cDNA indicated that about 50% of the cellular pool of pre-tRNA molecules for aspartic acid is edited. Thus, RNA editing occurs in mammalian mitochondria and can affect the anticodons of tRNAs.
RNA编辑已在原生动物和植物的细胞器基因中被描述，在哺乳动物的核转录本中也有三例被发现。有袋动物线粒体基因组中编码天冬氨酸的线粒体tRNA的反密码子为GCC。然而，该基因产物的cDNA显示，tRNA反密码子的第二个核苷酸在转录后发生变化，被识别为尿苷残基。这恢复了tRNA的编码特性。直接测序以及扩增cDNA的克隆表明，约50%的天冬氨酸前体tRNA分子细胞池被编辑。因此，RNA编辑发生在哺乳动物线粒体中，并可影响tRNA的反密码子。
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