着丝粒的结构域结构从裂殖酵母到人类都是保守的。
The domain structure of centromeres is conserved from fission yeast to humans.
作者信息
Kniola B, O'Toole E, McIntosh J R, Mellone B, Allshire R, Mengarelli S, Hultenby K, Ekwall K
机构信息
Karolinska Institutet, Department of Biosciences Novum/University College Sodertorn, Department of Natural Sciences, S-141 04 Huddinge, Sweden.
出版信息
Mol Biol Cell. 2001 Sep;12(9):2767-75. doi: 10.1091/mbc.12.9.2767.
Abstract
The centromeric DNA of fission yeast is arranged with a central core flanked by repeated sequences. The centromere-associated proteins, Mis6p and Cnp1p (SpCENP-A), associate exclusively with central core DNA, whereas the Swi6 protein binds the surrounding repeats. Here, electron microscopy and immunofluorescence light microscopy reveal that the central core and flanking regions occupy distinct positions within a heterochromatic domain. An "anchor" structure containing the Ndc80 protein resides between this heterochromatic domain and the spindle pole body. The organization of centromere-associated proteins in fission yeast is reminiscent of the multilayered structures of human kinetochores, indicating that such domain structure is conserved in eukaryotes.
摘要
裂殖酵母的着丝粒DNA由一个中央核心和两侧的重复序列组成。着丝粒相关蛋白Mis6p和Cnp1p（裂殖酵母中的CENP-A）仅与中央核心DNA结合，而Swi6蛋白则与周围的重复序列结合。在这里，电子显微镜和免疫荧光显微镜显示，中央核心和侧翼区域在异染色质域内占据不同的位置。一个包含Ndc80蛋白的“锚”结构位于这个异染色质域和纺锤极体之间。裂殖酵母中着丝粒相关蛋白的组织方式让人联想到人类动粒的多层结构，这表明这种结构域结构在真核生物中是保守的。
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