蒸汽灭菌后羟丙基甲基纤维素与双相磷酸钙之间的相互作用：毛细管气相色谱研究
Interaction between hydroxypropyl methylcellulose and biphasic calcium phosphate after steam sterilisation: capillary gas chromatography studies.
作者信息
Bourges X, Schmitt M, Amouriq Y, Daculsi G, Legeay G, Weiss P
机构信息
Centre de Transfert de Technologie du Mans, Département Matériaux, Le Mans, France.
出版信息
J Biomater Sci Polym Ed. 2001;12(6):573-9. doi: 10.1163/156856201316883412.
Abstract
The purpose of this study was to check the chemical stability of an injectable bone substitute (IBS) composed of a 50/50 w/w mixture of 2.92% hydroxypropyl methylcellulose (HPMC) solution in deionized water containing biphasic calcium phosphate (BCP) granules (60% hydroxyapatite/40% beta-tricalcium phosphate w/w). After separation of the organic and mineral phases, capillary gas chromatography (GC) was used to study the possible modification of HPMC due to the contact with BCP granules following steam sterilisation and 32 days storage at room temperature. HPMC was extracted from IBS in aqueous medium, and a dialytic method was then used to extract calcium phosphate salts from the HPMC. The percentage of HPMC extracted from BCP was 98.5%+/-0.5%, as measured by UV. GC showed no chemical modifications after steam sterilisation and storage.
摘要
本研究的目的是检测一种可注射骨替代物（IBS）的化学稳定性，该骨替代物由2.92%羟丙基甲基纤维素（HPMC）在去离子水中的50/50 w/w混合物与双相磷酸钙（BCP）颗粒（60%羟基磷灰石/40%β-磷酸三钙w/w）组成。在分离有机相和无机相后，采用毛细管气相色谱法（GC）研究蒸汽灭菌和室温储存32天后，由于与BCP颗粒接触，HPMC可能发生的改性。在水介质中从IBS中提取HPMC，然后采用透析法从HPMC中提取磷酸钙盐。通过紫外检测，从BCP中提取的HPMC百分比为98.5%±0.5%。气相色谱显示蒸汽灭菌和储存后没有化学改性。
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