Struillou Xavier, Rakic Mia, Badran Zahi, Macquigneau Laure, Colombeix Caroline, Pilet Paul, Verner Christian, Gauthier Olivier, Weiss Pierre, Soueidan Assem
Laboratory of Osteo-Articular and Dental Tissue Engineering (LIOAD), INSERM, U791, 1 Place Alexis Ricordeau, 44042, Nantes, France.
J Mater Sci Mater Med. 2013 Dec;24(12):2749-60. doi: 10.1007/s10856-013-5019-x. Epub 2013 Aug 3.
Hydrogel polymers have many applications in regenerative medicine. The aim of this study in dogs was to investigate bone regeneration in dehiscence-type peri-implant defects created surgically and treated with (i) biphasic calcium phosphate (BCP) granules alone; (ii) a composite putty hydroxypropyl methylcellulose (HPMC)/BCP (MBCP/putty); and (iii) a polymer crosslinked membrane of silanized-HPMC (Si-HPMC/BCP) compared with empty controls. At 3 months, new bone formation was significantly more important in defects filled with HPMC/BCP or Si-HPMC/BCP compared with spontaneous healing in control (P = 0.032 and P = 0.046 respectively) and more substantial compared with BCP alone. Furthermore, new bone formation in direct contact with the implant surface was observed in all three groups treated with BCP. The addition of HPMC to the BCP granules may have enhanced the initial stability of the material within the blood clot in these large and complex osseous defects. The Si-HPMC hydrogel may also act as an occlusive membrane covering the BCP, which could improve the stability of the granules in the defect area. However, the crosslinking time of the Si-HPMC is too long for easy handling and the mechanical properties remain to be improved. The composite MBCP/putty appears to be a valuable bone-graft material in complex defects in periodontology and implantology. These encouraging results should now be confirmed in clinical studies.
水凝胶聚合物在再生医学中有许多应用。本研究以犬为对象，旨在研究通过手术制造的裂开型种植体周围缺损的骨再生情况，并分别用以下方法进行治疗：（i）单独使用双相磷酸钙（BCP）颗粒；（ii）复合油灰状羟丙基甲基纤维素（HPMC）/BCP（MBCP/油灰）；（iii）硅烷化-HPMC（Si-HPMC/BCP）的聚合物交联膜，并与空白对照组进行比较。在3个月时，与对照组的自发愈合相比，填充HPMC/BCP或Si-HPMC/BCP的缺损处新骨形成明显更显著（分别为P = 0.032和P = 0.046），且比单独使用BCP时更大量。此外，在所有接受BCP治疗的三组中均观察到与种植体表面直接接触的新骨形成。在这些大而复杂的骨缺损中，向BCP颗粒中添加HPMC可能增强了材料在血凝块内的初始稳定性。Si-HPMC水凝胶也可能作为覆盖BCP的封闭膜，这可以提高颗粒在缺损区域的稳定性。然而，Si-HPMC的交联时间过长，不易操作，其机械性能仍有待改善。复合MBCP/油灰似乎是牙周病学和种植学复杂缺损中有价值的骨移植材料。这些令人鼓舞的结果现在应该在临床研究中得到证实。
