Komatsuzawa H, Ohta K, Fujiwara T, Choi G H, Labischinski H, Sugai M
Department of Microbiology, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8553, Japan.
FEMS Microbiol Lett. 2001 Sep 11;203(1):49-54. doi: 10.1111/j.1574-6968.2001.tb10819.x.
Two Tn551 insertional mutants with reduced methicillin resistance were isolated from methicillin-resistant Staphylococcus aureus KSA8. These two mutants showed increased susceptibility to beta-lactam antibiotics and bacitracin, but not to fosfomycin and vancomycin. Tn551 in these mutants was inserted into the same gene, termed fmtC. The fmtC gene has an open reading frame of 840 amino acid residues with an estimated molecular mass of 96.9 kDa. The N-terminal half of the deduced FmtC protein is very hydrophobic, implying that this protein is a membrane-associated protein.
从耐甲氧西林金黄色葡萄球菌KSA8中分离出两个耐甲氧西林能力降低的Tn551插入突变体。这两个突变体对β-内酰胺类抗生素和杆菌肽的敏感性增加，但对磷霉素和万古霉素不敏感。这些突变体中的Tn551插入到同一个基因中，该基因被称为fmtC。fmtC基因有一个840个氨基酸残基的开放阅读框，估计分子量为96.9 kDa。推导的FmtC蛋白的N端一半非常疏水，这意味着该蛋白是一种膜相关蛋白。
