Murakami H, Matsumaru H, Kanamori M, Hayashi H, Ohta T
College of Medical Technology and Nursing, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan.
Biochem Biophys Res Commun. 1999 Oct 22;264(2):348-51. doi: 10.1006/bbrc.1999.1388.
A novel gene, drp35, of Staphylococcus aureus, which was inducible especially with cell wall-affecting antibiotics, has been cloned. Analysis of differential hybridization with mRNAs enhanced in the presence of beta-lactams resulted in two positive clones that harbored a new gene encoding a 35,845-Da protein (Drp35) and the penicillin-binding protein 2 (PBP2). Immunoblot analysis revealed that the Drp35 protein band was evidently enhanced after 30 min in the presence of beta-lactams. The Drp35 expression was also enhanced with not only beta-lactams, but also vancomycin, bacitracin, and fosfomycin. Homology search revealed that Drp35 was a new protein. Our results revealed that it was specific in S. aureus and respondent to these agents in both methicillin-resistant and -sensitive strains of S. aureus.
已克隆出金黄色葡萄球菌的一个新基因drp35，该基因尤其可被影响细胞壁的抗生素诱导。对在β-内酰胺存在下增强的mRNA进行差异杂交分析，得到了两个阳性克隆，它们含有一个编码35845道尔顿蛋白质（Drp35）的新基因和青霉素结合蛋白2（PBP2）。免疫印迹分析显示，在β-内酰胺存在30分钟后，Drp35蛋白条带明显增强。Drp35的表达不仅在β-内酰胺作用下增强，在万古霉素、杆菌肽和磷霉素作用下也增强。同源性搜索显示Drp35是一种新蛋白质。我们的结果表明，它在金黄色葡萄球菌中具有特异性，并且在耐甲氧西林和甲氧西林敏感的金黄色葡萄球菌菌株中对这些药物均有反应。
