Glazer A N, Cohen-Bazire G
Arch Microbiol. 1975 Jun 20;104(1):29-32. doi: 10.1007/BF00447296.
The spectroscopically different phycocyanins present in the type strain of Hemiselmis virescens, Millport 64, and in a second strain of this cryptophytan species, Plymouth 157, have been purified and compared. They are similar in native molecular weight and in subunit structure, both containing alpha and beta subunits with molecular weights of approximately 10000 and 19000 respectively. However, they do not have the same chromophore composition. Both subunits of the phycocyanin of Plymouth 157 contain two bilins with absorption maxima at 600 and 660 nm, respectively. Only the beta subunit of Milllport 64 carries these chromophores; its alpha subunit bears another chromophore, with absorption maxima at 368 and 694 nm. The spectroscopic differences between the two native phycocyanins can be entirely accounted for by their differing chromophore compositions. The phycocyanin of Millport 64 is the only biliprotein so far described which contains three chemically different chromophores.
对绿半隐藻（Hemiselmis virescens）模式菌株Millport 64以及该隐藻物种的另一菌株Plymouth 157中光谱特性不同的藻蓝蛋白进行了纯化和比较。它们的天然分子量和亚基结构相似，均含有α和β亚基，分子量分别约为10000和19000。然而，它们的发色团组成不同。Plymouth 157的藻蓝蛋白的两个亚基均含有两种分别在600和660 nm处有最大吸收峰的藻胆素。Milllport 64只有β亚基携带这些发色团；其α亚基带有另一种发色团，在368和694 nm处有最大吸收峰。两种天然藻蓝蛋白之间的光谱差异完全可以由它们不同的发色团组成来解释。Millport 64的藻蓝蛋白是迄今为止所描述的唯一一种含有三种化学性质不同的发色团的双蛋白。
