Glazer A N, Bryant D A
Arch Microbiol. 1975 Jun 20;104(1):15-22. doi: 10.1007/BF00447294.
A hitherto undescribed red fluorescent phycobiliprotein (maximum emission at congruent to 680 nm), characterized by long wavelength absorption maxima in the visible region at 671 nm (xi = 172000 M(-1).cm(-1) per monomer of mol. wt. 30600)and 618 nm, has been purified to homogeneity from unicellular cyanobacterium, Synechococcus sp., and from a filamentous cyanobacterium, Anabaena variabilis. The name allophycocyanin B has been proposed for the new protein. A. variabilis allophycocyanin B is characterized by a native molecular weight of 89000 p 5000 (in 0.05 M phosphate at pH 7.2), an isoelectric point of 5.09, and a subunit molecular weight, based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, of 15300. The protein contains one phycocyanobilin chromophore per subunit. In common with allophycocyanin from the same organism, allophycocyanin B does not contain either histidine or tryptophan. In other respects, the amino acid compositions of the two proteins are significantly different. Synechococcus sp. (Anacytis nidulans) allophycocyanin B gives two components of 16000 and 17000 mol. wt., of equal staining intensity, on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Allophycocyanin B from both organisms cross-react with rabbit antisera directed against either Synechococcus sp. or Anabaena sp. allophycocyanin, but not with antisera against the phycocyanins of the same organisms. It is suggested that allophycocyanin B occupoes a position between allophycocyanin and chlorophyll a in the energy transfer path from the accessory pigments to species of chlorophyll a with absorption maxima at lambda greater than 670 nm.
一种迄今未被描述的红色荧光藻胆蛋白（最大发射波长约为680 nm），其特征在于在可见光区域具有长波长吸收最大值，分别位于671 nm（每摩尔质量为30600的单体的吸光系数为172000 M⁻¹·cm⁻¹）和618 nm，已从单细胞蓝细菌聚球藻属（Synechococcus sp.）和丝状蓝细菌多变鱼腥藻（Anabaena variabilis）中纯化至同质。已为这种新蛋白质提出了别藻蓝蛋白B这一名称。多变鱼腥藻别藻蓝蛋白B的特征在于其天然分子量为89000±5000（在pH 7.2的0.05 M磷酸盐中），等电点为5.09，基于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的亚基分子量为15300。该蛋白质每个亚基含有一个藻蓝胆素发色团。与来自同一生物体的别藻蓝蛋白一样，别藻蓝蛋白B既不含有组氨酸也不含有色氨酸。在其他方面，这两种蛋白质的氨基酸组成有显著差异。聚球藻属（Anacytis nidulans）别藻蓝蛋白B在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上产生两个分子量分别为16000和17000的组分，染色强度相同。来自这两种生物体的别藻蓝蛋白B都能与针对聚球藻属或鱼腥藻属别藻蓝蛋白的兔抗血清发生交叉反应，但不能与针对同一生物体藻蓝蛋白的抗血清发生反应。有人提出，在从辅助色素到最大吸收波长大于670 nm的叶绿素a物种的能量传递途径中，别藻蓝蛋白B位于别藻蓝蛋白和叶绿素a之间。
