Hackney J D, Evans M J, Christie B R
Aviat Space Environ Med. 1975 Jun;46(6):791-4.
The purpose of this study was to determine the effects on cell division of 60 and 80% oxygen in the lung alveoli of squirrel monkeys. To accomplish this, squirrel monkeys were exposed to 60 and 80% oxygen for up to 8 d. Prior to sacrifice, cells preparing to divide were labeled with tritiated thymidine (3H-TdR). Labeled cells were visualized with autoradiographic techinques, counted with the light microscope, and expressed in terms of a labeling index. In the present study, it was shown that DNA synthesis was not changed by exposure to 60%; however, with exposure to 80% oxygen, cell labeling was well above control levels by 5 d. Analysis of the cell types involved showed an increase in labeling. due to an increase in dividing Type 2 cells. Other cells in the alveolar wall also showed an increase in labeling
本研究的目的是确定松鼠猴肺泡中60%和80%的氧气对细胞分裂的影响。为实现这一目的，将松鼠猴暴露于60%和80%的氧气中长达8天。在处死前，用氚标记的胸腺嘧啶核苷（3H-TdR）标记准备分裂的细胞。用放射自显影技术观察标记细胞，在光学显微镜下计数，并以标记指数表示。在本研究中，结果表明，暴露于60%的氧气中不会改变DNA合成；然而，暴露于80%的氧气中，到第5天时细胞标记率远高于对照水平。对所涉及细胞类型的分析显示标记增加，这是由于分裂的Ⅱ型细胞增加所致。肺泡壁中的其他细胞标记也有所增加。
