Cagle P T, Langston C, Goodman J C, Thurlbeck W M
Department of Pathology, Baylor College of Medicine, Houston, TX 77030.
Am J Respir Cell Mol Biol. 1990 Aug;3(2):153-8. doi: 10.1165/ajrcmb/3.2.153.
To determine the extent and sequence of proliferation of the various types of cells in postpneumonectomy lung growth, 4-wk-old male Sprague-Dawley rats were injected with tritiated thymidine prior to sacrifice on days 2, 4, 7, and 10 after left pneumonectomy. Autoradiography of sections from each lobe of the remaining lung was performed and compared to controls. All of the cell types studied, both parenchymal and nonparenchymal, participated in postpneumonectomy lung growth. Mesothelial cell labeling peaked on day 2 and declined thereafter. Labeling of alveolar endothelial cells, alveolar interstitial cells, types I and II pneumonocytes, bronchial epithelium, and nonparenchymal endothelium occurred in parallel, peaking on day 4. The different lobes had equal percentages of dividing cells, suggesting that a global stimulus such as an endocrine factor has a dominant role in postpneumonectomy lung growth.
为了确定肺切除术后肺生长过程中各类细胞增殖的程度和顺序，在4周龄雄性Sprague-Dawley大鼠左肺切除术后第2、4、7和10天处死前，给它们注射了氚标记的胸腺嘧啶核苷。对剩余肺各叶的切片进行放射自显影，并与对照组进行比较。研究的所有细胞类型，包括实质细胞和非实质细胞，都参与了肺切除术后的肺生长。间皮细胞标记在第2天达到峰值，此后下降。肺泡内皮细胞、肺泡间质细胞、I型和II型肺上皮细胞、支气管上皮和非实质内皮细胞的标记平行出现，在第4天达到峰值。不同肺叶的分裂细胞百分比相等，这表明诸如内分泌因子等全身性刺激在肺切除术后的肺生长中起主导作用。
