Hackney J D, Evans M J, Spier C E
Aviat Space Environ Med. 1975 Nov;46(11):1340-2.
The purpose of this study was to determine the effects of 100% oxygen on cell division in lung alveoli of squirrel monkeys. To accomplish this, squirrel monkeys were exposed to 100% oxygen for up to 5 d. Prior to sacrifice, cells preparing to divide were labeled with tritiated thymidine (3H-Tdr). Labeled cells were visualized with autoradiographic techniques, counted with the light microscope, and expressed in terms of a labeling index. In the present study it was shown that DNA synthesis was initially inhibited by exposure to 100% oxygen. However, within 3 d it was returning to normal and by 5 d was well above control levels. Analysis of the cell types involved showed that the large increase in labeling was due to an increase in dividing Type 2 cells, which is thought to be for replacement of damaged Type 1 cells.
本研究的目的是确定100%氧气对松鼠猴肺泡细胞分裂的影响。为实现这一目的，将松鼠猴暴露于100%氧气中长达5天。在处死前，用氚标记的胸腺嘧啶核苷（3H-Tdr）标记准备分裂的细胞。用放射自显影技术观察标记细胞，用光学显微镜计数，并以标记指数表示。在本研究中发现，暴露于100%氧气最初会抑制DNA合成。然而，在3天内其恢复正常，到5天时已远高于对照水平。对所涉及细胞类型的分析表明，标记的大幅增加是由于分裂的Ⅱ型细胞增加，这被认为是为了替代受损的Ⅰ型细胞。
