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相似文献

1
Glycosylation of ribonuclease A catalysed by rabbit liver extracts.兔肝提取物催化的核糖核酸酶A的糖基化作用。
Biochem J. 1975 Feb;146(2):299-307. doi: 10.1042/bj1460299.
2
Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes.酵母细胞膜制剂催化的天冬酰胺序列的体外糖基化。
Biochem J. 1976 Oct 15;160(1):37-41. doi: 10.1042/bj1600037.
3
UDP-N-acetyl-D-glucosamine-asparagine sequon N-acetyl-beta-D-glucosaminyl-transferase-activity in human serum.人血清中UDP-N-乙酰-D-葡萄糖胺-天冬酰胺序列N-乙酰-β-D-葡萄糖胺基转移酶活性
Carbohydr Res. 1976 Jul;49:455-73. doi: 10.1016/s0008-6215(00)83163-8.
4
The UDP-N-acetylglucosamine-asparagine-sequon N-acetyl-beta-D-glucosaminyltransferase activity in preparations of rough endoplasmic reticulum from regenerating rat liver.来自再生大鼠肝脏的糙面内质网制剂中的UDP-N-乙酰葡糖胺-天冬酰胺序列N-乙酰-β-D-葡糖胺基转移酶活性
Biochem J. 1977 Apr 15;164(1):257-61. doi: 10.1042/bj1640257.
5
Properties of membrane-bound uridine diphosphate N-acetylglucosamine-asparagine sequon N-acetylglucosaminyltransferase in preparations of endoplasmic reticulum from rabbit liver and from regenerating rat liver [proceedings].兔肝脏及再生大鼠肝脏内质网制剂中膜结合的尿苷二磷酸N-乙酰葡糖胺-天冬酰胺糖基序N-乙酰葡糖胺基转移酶的性质[会议论文集]
Biochem Soc Trans. 1977;5(5):1337-40. doi: 10.1042/bst0051337.
6
Mammalian ribonucleases. The absence of a glycosylated Asn-Pro-Thr sequence in horse ribonuclease and the presence of tryptophan at position 39 in horse and dromedary ribonuclease.哺乳动物核糖核酸酶。马核糖核酸酶中不存在糖基化的天冬酰胺-脯氨酸-苏氨酸序列，以及马和单峰驼核糖核酸酶中第39位存在色氨酸。
FEBS Lett. 1985 Jun 3;185(1):115-20. doi: 10.1016/0014-5793(85)80752-3.
7
The control of glycoprotein synthesis: N-acetylglucosamine linkage to a mannose residue as a signal for the attachment of L-fucose to the asparagine-linked N-acetylglucosamine residue of glycopeptide from alpha1-acid glycoprotein.糖蛋白合成的调控：N-乙酰葡糖胺与甘露糖残基的连接作为L-岩藻糖连接至源自α1-酸性糖蛋白的糖肽中天冬酰胺连接的N-乙酰葡糖胺残基的信号。
Biochem Biophys Res Commun. 1976 Oct 4;72(3):909-16. doi: 10.1016/s0006-291x(76)80218-5.
8
Attachment of terminal N-acetylglucosamine to asparagine-linked oligosaccharides occurs in central cisternae of the Golgi stack.末端N-乙酰葡糖胺与天冬酰胺连接的寡糖的连接发生在高尔基体堆叠的中央潴泡中。
Cell. 1985 Feb;40(2):463-72. doi: 10.1016/0092-8674(85)90161-8.
9
Stimulation by GTP of the incorporation of N-acetylglucosamine and mannose into endogenous acceptors of rough endoplasmic reticulum in rat liver.鸟苷三磷酸（GTP）对大鼠肝脏粗面内质网内源性受体中N - 乙酰葡糖胺和甘露糖掺入的刺激作用。
Arch Int Physiol Biochim. 1976;84(3):624-5.
10
Oligosaccharyl transferase is a constitutive component of an oligomeric protein complex from pig liver endoplasmic reticulum.寡糖基转移酶是猪肝内质网中一种寡聚蛋白复合物的组成成分。
Eur J Biochem. 1995 Mar 15;228(3):689-96.

引用本文的文献

1
Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity.1型核糖体失活蛋白的结构分析揭示了多个L-天冬酰胺-N-乙酰-D-葡糖胺单糖修饰：对细胞毒性的影响。
Mol Med Rep. 2015 Oct;12(4):5737-45. doi: 10.3892/mmr.2015.4146. Epub 2015 Jul 29.
2
Protein quality control in the early secretory pathway.早期分泌途径中的蛋白质质量控制
EMBO J. 2008 Jan 23;27(2):315-27. doi: 10.1038/sj.emboj.7601974.
3
Specific RNase isoenzymes in the human central nervous system.人类中枢神经系统中的特定核糖核酸酶同工酶。
Neurochem Res. 1987 Dec;12(12):1067-76. doi: 10.1007/BF00971706.
4
The UDP-N-acetylglucosamine-asparagine-sequon N-acetyl-beta-D-glucosaminyltransferase activity in preparations of rough endoplasmic reticulum from regenerating rat liver.来自再生大鼠肝脏的糙面内质网制剂中的UDP-N-乙酰葡糖胺-天冬酰胺序列N-乙酰-β-D-葡糖胺基转移酶活性
Biochem J. 1977 Apr 15;164(1):257-61. doi: 10.1042/bj1640257.
5
Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes.酵母细胞膜制剂催化的天冬酰胺序列的体外糖基化。
Biochem J. 1976 Oct 15;160(1):37-41. doi: 10.1042/bj1600037.
6
Enzymatic conversion of proteins to glycoproteins.蛋白质的酶促转化为糖蛋白。
Proc Natl Acad Sci U S A. 1977 Jan;74(1):134-8. doi: 10.1073/pnas.74.1.134.

本文引用的文献

1
[Purification of trypsinogen and bovine trypsin by chromatography on carboxymethyl cellulose].通过羧甲基纤维素柱色谱法纯化胰蛋白酶原和牛胰蛋白酶
Biochim Biophys Acta. 1962 Jan 1;56:202-4. doi: 10.1016/0006-3002(62)90556-5.
2
ON THE STRUCTURE OF BOVINE PANCREATIC RIBONUCLEASE B. ISOLATION OF A GLYCOPEPTIDE.关于牛胰核糖核酸酶B的结构。一种糖肽的分离。
J Biol Chem. 1964 Aug;239:2530-8.
3
STUDIES ON THE STRUCTURAL AND ENZYMIC ORGANIZATION OF THE MEMBRANOUS ELEMENTS OF LIVER MICROSOMES.肝脏微粒体膜性成分的结构与酶组织研究
Acta Pathol Microbiol Scand Suppl (1926). 1963:SUPPL166:1-94.
4
The sequence of amino acid residues in bovine pancreatic ribonuclease: revisions and confirmations.牛胰核糖核酸酶中氨基酸残基的序列：修订与确认
J Biol Chem. 1963 Jan;238:227-34.
5
Analytical separations by highvoltage paper electrophoresis. Amino acids in protein hydrolysates.高压纸电泳分析分离。蛋白质水解物中的氨基酸。
Biochem J. 1961 Dec;81(3):606-14. doi: 10.1042/bj0810606.
6
Chromatography of ribonuclease on carboxymethyl cellulose columns.核糖核酸酶在羧甲基纤维素柱上的色谱分析。
J Biol Chem. 1959 Oct;234:2652-6.
7
[Some factors which affect the enzymatic digestion of ribonucleic acid].[影响核糖核酸酶促消化的一些因素]
J Biol Chem. 1959 Jun;234(6):1512-6.
8
Attachment of glucosamine to protein at the ribosomal site of rat liver.氨基葡萄糖在大鼠肝脏核糖体部位与蛋白质的附着。
Biochemistry. 1967 Jul;6(7):1941-7. doi: 10.1021/bi00859a009.
9
Biosynthesis of glycoprotein by liver. The incorporation in vivo of 14C-glucosamine into protein-bound hexosamine and sialic acid of rat liver subcellular fractions.肝脏中糖蛋白的生物合成。大鼠肝脏亚细胞组分中14C-葡糖胺在体内掺入蛋白质结合的己糖胺和唾液酸的情况。
J Biol Chem. 1966 Nov 25;241(22):5408-18.
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The carbohydrate moiety of bovine pancreatic deoxyribonuclease.牛胰脱氧核糖核酸酶的碳水化合物部分
J Biol Chem. 1969 Feb 10;244(3):933-6.

兔肝提取物催化的核糖核酸酶A的糖基化作用。

Glycosylation of ribonuclease A catalysed by rabbit liver extracts.

作者信息

Khalkhall Z, Marshall R D

出版信息

Biochem J. 1975 Feb;146(2):299-307. doi: 10.1042/bj1460299.

DOI:10.1042/bj1460299
PMID:1156375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165306/
Abstract

Crude extracts of rabbit liver catalyse in vitro the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to bovine pancreatic ribonuclease A. The enzymic activity is contained in rough endoplasmic reticulum. It has an absolute requirement for a bivalent metal ion: Co-2+ greater than Mn-2+ greater than Ni-2+. Mg-2+ is ineffective. There is enzymic activity in the absence of detergent, but increased activity is observed in the presence of Triton X-100. The site of glycosylation of ribonuclease A is asparagine-34, and glycosylation occurs only at this point. These findings agree with the hypothesis that the sequence Asn-X-Thr(Ser) (where X may be one of a number of types of amino acid) is a necessary, but not sufficient, condition for N-acetylglucosaminylation of a protein-bound asparagine residue.

摘要

兔肝粗提物在体外催化N-乙酰葡糖胺从UDP-N-乙酰葡糖胺转移至牛胰核糖核酸酶A。酶活性存在于糙面内质网中。它对二价金属离子有绝对需求：Co²⁺＞Mn²⁺＞Ni²⁺。Mg²⁺无效。在没有去污剂的情况下存在酶活性，但在Triton X-100存在时观察到活性增加。核糖核酸酶A的糖基化位点是天冬酰胺-34，且糖基化仅在此位点发生。这些发现与以下假说相符，即序列Asn-X-Thr（Ser）（其中X可能是多种氨基酸类型之一）是蛋白质结合的天冬酰胺残基进行N-乙酰葡糖胺化的必要但不充分条件。