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酵母细胞膜制剂催化的天冬酰胺序列的体外糖基化。

Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes.

作者信息

Khalkhali Z, Marshall R D, Reuvers F, Habets-Willems C, Boer P

出版信息

Biochem J. 1976 Oct 15;160(1):37-41. doi: 10.1042/bj1600037.

DOI:10.1042/bj1600037
PMID:795426
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164198/
Abstract

Preparations of yeast cell membranes can catalyse in vitro the N-acetyl-beta-D-glucosaminylation of the asparagine sequon at residues 34--36 of bovine pancreatic ribonuclease A. The relevant glycopeptides were isolated from tryptic hydrolysates of the glycosylated ribonuclease and analysed. The donor used was UDP-N-acetyl-D-glucosamine, although the mechanism of the transfer is unknown. Mn2+ ions at concentrations of 25 mM double the activity of the enzymic transfer.

摘要

酵母细胞膜制剂能够在体外催化牛胰核糖核酸酶A第34 - 36位残基处天冬酰胺序列的N - 乙酰 - β - D - 葡糖胺化反应。从糖基化核糖核酸酶的胰蛋白酶水解产物中分离出相关糖肽并进行分析。尽管转移机制尚不清楚，但所用的供体是UDP - N - 乙酰 - D - 葡糖胺。浓度为25 mM的Mn2 +离子可使酶促转移活性加倍。

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引用本文的文献

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2
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Biochem J. 1977 Apr 15;164(1):257-61. doi: 10.1042/bj1640257.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Structure and function of ribonuclease.核糖核酸酶的结构与功能。
Adv Enzymol Relat Subj Biochem. 1962;24:161-261. doi: 10.1002/9780470124888.ch4.
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[Purification of trypsinogen and bovine trypsin by chromatography on carboxymethyl cellulose].通过羧甲基纤维素柱色谱法纯化胰蛋白酶原和牛胰蛋白酶
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BIOSYNTHESIS OF GLYCOPROTEINS. IV. THE SUBCELLULAR SITES OF INCORPORATION OF GLUCOSAMINE-1-14-C INTO GLYCOPROTEIN RAT LIVER.糖蛋白的生物合成。IV. 葡糖胺-1-¹⁴C掺入大鼠肝脏糖蛋白的亚细胞定位
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Chromatography of ribonuclease on carboxymethyl cellulose columns.核糖核酸酶在羧甲基纤维素柱上的色谱分析。
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Biosynthesis of glycoprotein by liver. The incorporation in vivo of 14C-glucosamine into protein-bound hexosamine and sialic acid of rat liver subcellular fractions.肝脏中糖蛋白的生物合成。大鼠肝脏亚细胞组分中14C-葡糖胺在体内掺入蛋白质结合的己糖胺和唾液酸的情况。
J Biol Chem. 1966 Nov 25;241(22):5408-18.
8
The secretory pathways of rat serum glycoproteins and albumin. Localization of newly formed proteins within the endoplasmic reticulum.大鼠血清糖蛋白和白蛋白的分泌途径。内质网内新形成蛋白质的定位。
J Cell Biol. 1972 Feb;52(2):231-45. doi: 10.1083/jcb.52.2.231.
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Studies on the site of biosynthesis of acidic glycoproteins of guinea-pig serum.豚鼠血清酸性糖蛋白生物合成部位的研究。
Biochem J. 1967 Apr;103(1):153-64. doi: 10.1042/bj1030153.
10
Identification of asparagine as the site of glycosylation in glycoprotein biosynthesis.确定天冬酰胺为糖蛋白生物合成中糖基化的位点。
J Biochem. 1973 May;73(5):1089-94. doi: 10.1093/oxfordjournals.jbchem.a130163.