酵母细胞膜制剂催化的天冬酰胺序列的体外糖基化。
Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes.
作者信息
Khalkhali Z, Marshall R D, Reuvers F, Habets-Willems C, Boer P
出版信息
Biochem J. 1976 Oct 15;160(1):37-41. doi: 10.1042/bj1600037.
Abstract
Preparations of yeast cell membranes can catalyse in vitro the N-acetyl-beta-D-glucosaminylation of the asparagine sequon at residues 34--36 of bovine pancreatic ribonuclease A. The relevant glycopeptides were isolated from tryptic hydrolysates of the glycosylated ribonuclease and analysed. The donor used was UDP-N-acetyl-D-glucosamine, although the mechanism of the transfer is unknown. Mn2+ ions at concentrations of 25 mM double the activity of the enzymic transfer.
摘要
酵母细胞膜制剂能够在体外催化牛胰核糖核酸酶A第34 - 36位残基处天冬酰胺序列的N - 乙酰 - β - D - 葡糖胺化反应。从糖基化核糖核酸酶的胰蛋白酶水解产物中分离出相关糖肽并进行分析。尽管转移机制尚不清楚，但所用的供体是UDP - N - 乙酰 - D - 葡糖胺。浓度为25 mM的Mn2 +离子可使酶促转移活性加倍。
相似文献
1
Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes.酵母细胞膜制剂催化的天冬酰胺序列的体外糖基化。
Biochem J. 1976 Oct 15;160(1):37-41. doi: 10.1042/bj1600037.
2
UDP-N-acetyl-D-glucosamine-asparagine sequon N-acetyl-beta-D-glucosaminyl-transferase-activity in human serum.人血清中UDP-N-乙酰-D-葡萄糖胺-天冬酰胺序列N-乙酰-β-D-葡萄糖胺基转移酶活性
Carbohydr Res. 1976 Jul;49:455-73. doi: 10.1016/s0008-6215(00)83163-8.
3
Glycosylation of ribonuclease A catalysed by rabbit liver extracts.兔肝提取物催化的核糖核酸酶A的糖基化作用。
Biochem J. 1975 Feb;146(2):299-307. doi: 10.1042/bj1460299.
4
The UDP-N-acetylglucosamine-asparagine-sequon N-acetyl-beta-D-glucosaminyltransferase activity in preparations of rough endoplasmic reticulum from regenerating rat liver.来自再生大鼠肝脏的糙面内质网制剂中的UDP-N-乙酰葡糖胺-天冬酰胺序列N-乙酰-β-D-葡糖胺基转移酶活性
Biochem J. 1977 Apr 15;164(1):257-61. doi: 10.1042/bj1640257.
5
A simple and efficient method to remove ribonuclease contamination from pancreatic deoxyribonuclease preparations.一种从胰腺脱氧核糖核酸酶制剂中去除核糖核酸酶污染的简单高效方法。
Anal Biochem. 1976 Oct;75(2):402-9. doi: 10.1016/0003-2697(76)90094-4.
6
The structural basis of the difference in sensitivity for PNGase F in the de-N-glycosylation of the native bovine pancreatic ribonucleases B and BS.天然牛胰核糖核酸酶B和BS去N-糖基化过程中对PNGase F敏感性差异的结构基础。
Biochemistry. 2008 Mar 18;47(11):3435-46. doi: 10.1021/bi7012504. Epub 2008 Feb 23.
7
Effect of C-domain N-glycosylation and deletion on rat pancreatic alpha-amylase secretion and activity.C 结构域 N-糖基化和缺失对大鼠胰腺α-淀粉酶分泌及活性的影响。
Biochem J. 2002 Feb 15;362(Pt 1):259-64. doi: 10.1042/0264-6021:3620259.
8
Formation of lipid-bound N-acetylhexosamine derivatives in yeast particulate fractions.酵母颗粒组分中脂质结合的N-乙酰己糖胺衍生物的形成。
Acta Biochim Pol. 1976;23(4):357-67.
9
Structure of a complex asparagine bound glycopeptide from horse pancreatic ribonuclease containing (2 leads to 3) and (2 leads to 6) linked sialic acid residues.来自马胰腺核糖核酸酶的含有（2→3）和（2→6）连接唾液酸残基的复杂天冬酰胺结合糖肽的结构
Biochem Biophys Res Commun. 1978 Jun 29;82(4):1223-8. doi: 10.1016/0006-291x(78)90318-2.
10
Effect of reductive lactosamination on the hepatic uptake of bovine pancreatic ribonuclease A dimer.还原性乳糖胺化对牛胰核糖核酸酶A二聚体肝脏摄取的影响。
J Biol Chem. 1978 Apr 10;253(7):2070-2.
引用本文的文献
1
Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity.1型核糖体失活蛋白的结构分析揭示了多个L-天冬酰胺-N-乙酰-D-葡糖胺单糖修饰：对细胞毒性的影响。
Mol Med Rep. 2015 Oct;12(4):5737-45. doi: 10.3892/mmr.2015.4146. Epub 2015 Jul 29.
2
The UDP-N-acetylglucosamine-asparagine-sequon N-acetyl-beta-D-glucosaminyltransferase activity in preparations of rough endoplasmic reticulum from regenerating rat liver.来自再生大鼠肝脏的糙面内质网制剂中的UDP-N-乙酰葡糖胺-天冬酰胺序列N-乙酰-β-D-葡糖胺基转移酶活性
Biochem J. 1977 Apr 15;164(1):257-61. doi: 10.1042/bj1640257.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Structure and function of ribonuclease.核糖核酸酶的结构与功能。
Adv Enzymol Relat Subj Biochem. 1962;24:161-261. doi: 10.1002/9780470124888.ch4.
3
[Purification of trypsinogen and bovine trypsin by chromatography on carboxymethyl cellulose].通过羧甲基纤维素柱色谱法纯化胰蛋白酶原和牛胰蛋白酶
Biochim Biophys Acta. 1962 Jan 1;56:202-4. doi: 10.1016/0006-3002(62)90556-5.
4
5
Chromatography of ribonuclease on carboxymethyl cellulose columns.核糖核酸酶在羧甲基纤维素柱上的色谱分析。
J Biol Chem. 1959 Oct;234:2652-6.
6
The effect of guanosine diphosphate and triphosphate on the incorporation of labeled amino acids into proteins.二磷酸鸟苷和三磷酸鸟苷对标记氨基酸掺入蛋白质的影响。
J Biol Chem. 1956 Jul;221(1):45-59.
7
8
The secretory pathways of rat serum glycoproteins and albumin. Localization of newly formed proteins within the endoplasmic reticulum.大鼠血清糖蛋白和白蛋白的分泌途径。内质网内新形成蛋白质的定位。
J Cell Biol. 1972 Feb;52(2):231-45. doi: 10.1083/jcb.52.2.231.
9
Studies on the site of biosynthesis of acidic glycoproteins of guinea-pig serum.豚鼠血清酸性糖蛋白生物合成部位的研究。
Biochem J. 1967 Apr;103(1):153-64. doi: 10.1042/bj1030153.
10
Identification of asparagine as the site of glycosylation in glycoprotein biosynthesis.确定天冬酰胺为糖蛋白生物合成中糖基化的位点。
J Biochem. 1973 May;73(5):1089-94. doi: 10.1093/oxfordjournals.jbchem.a130163.
Zotero 插件