McIlwain K L, Merriweather M Y, Yuva-Paylor L A, Paylor R
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Physiol Behav. 2001 Aug;73(5):705-17. doi: 10.1016/s0031-9384(01)00528-5.
Our laboratory uses a specific test battery for the initial assessment of phenotypic behavioral differences of transgenic, knockout, and inbred strains of mice. Our standard battery includes: open field activity, light-dark exploration, rotarod, prepulse inhibition (PPI), acoustic startle habituation, conditioned fear, Morris water maze, and hot plate. Tests are run in the order listed, from least invasive to most invasive, to decrease the chance that behavioral responses are altered by prior test history. The studies presented here were designed around two questions. The first study asks if differences exist between mice that have undergone testing on different tasks and mice that are naïve to the test experience. The second study asks if the test order affects how an animal performs on subsequent tests. In the first experiment, C57BL/6J male mice were evaluated on all of the tests described above. The behavior of these 'test battery' mice was compared to aged matched naïve mice that were only tested on one test from the battery. Results indicate that on some tests, the behavior of 'test battery' mice was significantly different from the behavior of naïve mice, while on other tests there were no differences. For example, test battery mice responded differently in the open-field, rotarod, and hot-plate test, but behaved similar on the PPI and conditioned fear. Experiments in the second study were performed on male 129/SvEvTac (129S6) and C57BL/6J male mice. An abbreviated battery of tasks was used and the results suggest that certain test variables are sensitive to test order, whereas others are resistant. These two studies demonstrate that some behavioral tests appear to be sensitive to previous testing experience, while other tests are immune.
我们实验室使用特定的测试组合对转基因、基因敲除和近交系小鼠的表型行为差异进行初步评估。我们的标准测试组合包括：旷场活动、明暗箱探索、转棒试验、前脉冲抑制（PPI）、听觉惊吓习惯化、条件性恐惧、莫里斯水迷宫和热板试验。测试按照列出的顺序进行，从侵入性最小到侵入性最大，以减少行为反应因先前测试经历而改变的可能性。这里呈现的研究围绕两个问题设计。第一项研究询问在不同任务上接受过测试的小鼠与未经历过测试的天真小鼠之间是否存在差异。第二项研究询问测试顺序是否会影响动物在后续测试中的表现。在第一个实验中，对C57BL/6J雄性小鼠进行了上述所有测试。将这些“测试组合”小鼠的行为与仅在测试组合中的一项测试上接受过测试的年龄匹配的天真小鼠进行比较。结果表明，在某些测试中，“测试组合”小鼠的行为与天真小鼠的行为有显著差异，而在其他测试中则没有差异。例如，测试组合小鼠在旷场、转棒和热板测试中的反应不同，但在PPI和条件性恐惧测试中的表现相似。第二项研究的实验是在雄性129/SvEvTac（129S6）和C57BL/6J雄性小鼠上进行的。使用了一组简化的任务，结果表明某些测试变量对测试顺序敏感，而其他变量则不受影响。这两项研究表明，一些行为测试似乎对先前的测试经历敏感，而其他测试则不受影响。
