Witte D, Chirala M, Younes A, Li Y, Younes M
Department of Pathology, Baylor College of Medicine and the Methodist Hospital, Houston, TX 77030, USA.
Hum Pathol. 2001 Sep;32(9):940-4. doi: 10.1053/hupa.2001.27117.
Estrogen receptor beta (ER-beta) has recently been detected in a human colon cancer cell line. The aim of this work was to determine whether ER-beta is expressed in human colorectal carcinoma (CRC) tissue and the extent of this expression. ER-beta expression in CRC was investigated by immunohistochemical staining of sections of formalin-fixed, paraffin-embedded tissue from 55 CRC. The percent of positive cells was recorded. ER-beta immunoreactivity was always present in normal epithelium and adenomas in the same sections of some CRC and was always nuclear. In CRC, nuclear ER-beta immunoreactivity was detected in >10% of the cancer cells in 67% of the cases and was almost always associated with cytoplasmic immunoreactivity. There were no statistically significant differences between the ER-beta-positive and -negative groups in regard to depth of invasion, nodal metastases, or survival, regardless of the cut-off value used. We conclude that (1) a significant number of CRCs are positive for ER-beta. (2) estrogen may play an important role in the proliferation of normal colonic epithelium, and (3) there is differential localization of ER-beta immunoreactivity between normal colon, adenomas, and CRCs. Whether different ER-beta isoforms are differentially expressed in CRCs, and whether human CRCs respond to treatment with antiestrogens, is the subject of studies currently in progress.
最近在一种人类结肠癌细胞系中检测到雌激素受体β（ER-β）。本研究的目的是确定ER-β是否在人类结直肠癌（CRC）组织中表达以及这种表达的程度。通过对55例CRC患者福尔马林固定、石蜡包埋组织切片进行免疫组织化学染色，研究CRC中ER-β的表达情况。记录阳性细胞的百分比。在一些CRC的同一切片中，正常上皮和腺瘤中总是存在ER-β免疫反应性，且总是位于细胞核内。在CRC中，67%的病例中超过10%的癌细胞检测到细胞核ER-β免疫反应性，并且几乎总是与细胞质免疫反应性相关。无论使用何种临界值，ER-β阳性和阴性组在浸润深度、淋巴结转移或生存率方面均无统计学显著差异。我们得出结论：（1）相当数量的CRC对ER-β呈阳性。（2）雌激素可能在正常结肠上皮的增殖中起重要作用，以及（3）正常结肠、腺瘤和CRC之间ER-β免疫反应性存在不同定位。不同的ER-β亚型在CRC中是否差异表达，以及人类CRC是否对抗雌激素治疗有反应，是目前正在进行的研究课题。
