Younes Mamoun, Ly Charles J, Singh Kanchan, Ertan Atilla, Younes Pamela S, Bailey Jennifer M
Departments of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX, USA.
Department of Medicine, Section of Gastroenterology, Hepatology and Nutrition, University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX, USA.
Oncotarget. 2018 Dec 28;9(102):37715-37720. doi: 10.18632/oncotarget.26503.
Limited studies have shown that some patients with pancreatic adenocarcinoma (PAC) may benefit from treatment with tamoxifen. PAC has been shown to be largely negative for estrogen receptor alpha (ER-alpha). The aim of this pilot study was to investigate ER-beta expression in human PAC. Sections of tissue microarray with 18 evaluable cases of human PAC were stained by immunohistochemistry (IHC) for ER-beta1, ER-beta2, ER-beta5, and Cyclin A. The levels of ER-beta isoform expression and the S-phase fraction (SPF) were determined using quantitative digital image analysis. Higher mean and median ER-beta2 levels correlated with male sex ( = 0.057 and = 0.035, respectively), older age ( = 0.005 and = 0.006, respectively), and lower pT stage ( = 0.008 and = 0.009). Mean and median ER-beta5 levels correlated negatively with SPF ( = 0.021 and = 0.047, respectively). Mean ER-beta1 expression did not correlate with any of the above mentioned clinicopathologic factors. The findings in this pilot study, although should be considered preliminary, suggest that some ER-beta isoforms may play a role in the biology of PAC. Additional larger studies are needed to confirm our findings, and to determine whether ER-beta may be considered for future targeted therapy.
有限的研究表明，一些胰腺腺癌（PAC）患者可能从他莫昔芬治疗中获益。已证明PAC在很大程度上雌激素受体α（ER-α）呈阴性。本初步研究的目的是调查人PAC中ER-β的表达情况。对包含18例可评估的人PAC组织微阵列切片进行免疫组织化学（IHC）染色，检测ER-β1、ER-β2、ER-β5和细胞周期蛋白A。使用定量数字图像分析确定ER-β亚型表达水平和S期分数（SPF）。较高的ER-β2平均水平和中位数水平分别与男性（分别为 = 0.057和 = 0.035）、老年（分别为 = 0.005和 = 0.006）以及较低的pT分期（分别为 = 0.008和 = 0.009）相关。ER-β5的平均水平和中位数水平与SPF呈负相关（分别为 = 0.021和 = 0.047）。ER-β1的平均表达与上述任何临床病理因素均无相关性。本初步研究的结果虽然应被视为初步的，但表明一些ER-β亚型可能在PAC生物学中发挥作用。需要更多更大规模的研究来证实我们的发现，并确定ER-β是否可被考虑用于未来的靶向治疗。
