Tavian M, Robin C, Coulombel L, Péault B
INSERM U506, Hôpital Paul Brousse, 94807 Villejuif Cedex, France.
Immunity. 2001 Sep;15(3):487-95. doi: 10.1016/s1074-7613(01)00193-5.
We have traced emerging hematopoietic cells along human early ontogeny by culturing embryonic tissue rudiments in the presence of stromal cells that promote myeloid and B cell differentiation, and by assaying T cell potential in the NOD-SCID mouse thymus. Hematogenous potential was present inside the embryo as early as day 19 of development in the absence of detectable CD34+ hematopoietic cells, and spanned both lymphoid and myeloid lineages from day 24 in the splanchnopleural mesoderm and derived aorta where CD34+ progenitors appear at day 27. By contrast, hematopoietic cells arising in the third week yolk sac, as well as their progeny at later stages, were restricted to myelopoiesis and therefore are unlikely to contribute to definitive hematopoiesis in man.
我们通过在促进髓系和B细胞分化的基质细胞存在的情况下培养胚胎组织原基，并通过检测NOD-SCID小鼠胸腺中的T细胞潜能，追踪了人类早期个体发育过程中新兴的造血细胞。早在发育第19天，在未检测到CD34+造血细胞的情况下，胚胎内部就存在造血潜能，从第24天开始，在内脏中胚层和衍生的主动脉中，造血潜能涵盖了淋巴系和髓系谱系，而CD34+祖细胞在第27天出现。相比之下，在第三周卵黄囊中产生的造血细胞及其后期的后代仅限于髓系造血，因此不太可能对人类的确定性造血有贡献。
