Cumano A, Ferraz J C, Klaine M, Di Santo J P, Godin I
Unité du Développement des Lymphocytes (URA CNRS 1961), Institut Pasteur, Rue du Dr. Roux, 75724 Paris, France.
Immunity. 2001 Sep;15(3):477-85. doi: 10.1016/s1074-7613(01)00190-x.
The relative contribution of yolk sac and intraembryonic precursors to hematopoiesis has been a matter of long-standing controversy. As reconstitution activity has so far only been found in embryonic tissues after the onset of circulation, the origin of reconstituting cells could not be formally established. Here, we separated yolk sac and intraembryonic splanchnopleura prior to circulation and maintained the explants in organ culture before transfer. Precursors derived from the intraembryonic site generated multilineage hematopoietic progeny in adult mice for more than 6 months. Yolk sac cells only provided myeloid short-term reconstitution. The results reveal a differential hematopoietic capacity of precirculation embryonic tissues in vivo, and indicate that the only cells capable of adult long-term hematopoiesis are of intraembryonic origin.
卵黄囊和胚胎内造血前体细胞对造血作用的相对贡献一直是长期存在争议的问题。由于迄今为止仅在循环开始后的胚胎组织中发现了重建活性，因此无法正式确定重建细胞的起源。在此，我们在循环开始前分离了卵黄囊和胚胎内脏壁，并在转移前将外植体维持在器官培养中。源自胚胎内部位的前体细胞在成年小鼠中产生多谱系造血后代超过6个月。卵黄囊细胞仅提供髓系短期重建。结果揭示了体内循环前胚胎组织的造血能力差异，并表明唯一能够进行成年长期造血的细胞起源于胚胎内。
