Takita J, Katohda S, Sugiyama H
Nitto Best Co. Ltd., Sagae 991-8610, Japan.
Carbohydr Res. 2001 Sep 28;335(2):133-9. doi: 10.1016/s0008-6215(01)00215-4.
The structure of the title mannan was determined exclusively by NMR. Because of the short relaxation time of the native mannan (100 kDa), a partially hydrolyzed mannan (10 kDa) was used for proton assignments by COSY, to correlate proton and carbon signals by HMQC, and to determine linkage positions between residues by HMBC. A further hydrolyzed mannan (oligomers of approximately 1.5 kDa) was used to determine the anomeric configuration, using Wilker's quasi-3D method [Wilker, W.; Leibfritz, D. Magn. Reson. Chem. 1995, 33, 632-638]. The procedure presented here can be used to determine the structure of any polysaccharide.
标题中甘露聚糖的结构完全由核磁共振（NMR）确定。由于天然甘露聚糖（100 kDa）的弛豫时间较短，因此使用部分水解的甘露聚糖（10 kDa）通过COSY进行质子归属，通过HMQC关联质子和碳信号，并通过HMBC确定残基之间的连接位置。使用威尔克的准3D方法[威尔克，W.；莱布弗里茨，D.《磁共振化学》1995年，33卷，632 - 638页]，进一步水解的甘露聚糖（约1.5 kDa的低聚物）用于确定异头构型。这里介绍的方法可用于确定任何多糖的结构。
