Bystrický Peter, Machová Eva, Bystrický Slavomír
Division of Neurosciences, Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Eur Biophys J. 2018 Jul;47(5):591-596. doi: 10.1007/s00249-018-1298-y. Epub 2018 Apr 13.
A change from a globular to a filamentous hyphal form is an important feature in the pathogenicity of yeasts. Such a dimorphism while infecting a host organism is thought to be also accompanied in the case of Candida albicans spp. by a structural rearrangement of surface mannan antigen. The presented work brings new insights into the molecular structural changes of mannan C. albicans serotype B based on NMR experimental data. H and C signal identification of the anomeric region and the assignment of their linkage type is presented here. 2D deconvolution of the HSQC spectra facilitated accurate integration of all anomeric cross-peaks. Analysis of the differences in the integrals led to the proposal that C. albicans serotype B hyphal mannan side chains have the shortened structural moieties: Manα1-2Manα1- and Manα1-3 [Manα1-6] Manα1-2Manα1-. These represent the dominant structures important for construction of a saccharide-based prospective anti-candida vaccine.
从球状转变为丝状菌丝形态是酵母致病性的一个重要特征。在感染宿主生物体时，这种二态性被认为在白色念珠菌属的情况下还伴随着表面甘露聚糖抗原的结构重排。本文基于核磁共振实验数据，对B型白色念珠菌甘露聚糖的分子结构变化带来了新的见解。这里展示了端基异构区域的氢和碳信号识别及其连接类型的归属。HSQC谱的二维去卷积有助于所有端基异构交叉峰的准确积分。对积分差异的分析表明，B型白色念珠菌菌丝甘露聚糖侧链具有缩短的结构部分：Manα1-2Manα1-和Manα1-3[Manα1-6]Manα1-2Manα1-。这些代表了构建基于糖类的抗念珠菌疫苗的重要主导结构。
