Matsuo K, Isogai E, Araki Y
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
J Clin Microbiol. 2000 Oct;38(10):3750-4. doi: 10.1128/JCM.38.10.3750-3754.2000.
Previously, Rhodotorula glutinis was reported to produce a large amount of exocellular mannan, having a repeating unit of -->3)-D-Manp-(1-->4)-D-Manp-(1-->. Recently, we found that antigenic polysaccharides of Leptospira biflexa serovar patoc strain Patoc I have the same repeating unit and cross-react with antisera raised against extended strains of other leptospires (K. Matsuo, E. Isogai, and Y. Araki, Carbohydr. Res., in press). This structural identity and the difficulty of producing and isolating antigens led us to confirm the usefulness of Rhodotorula mannan as an immunoreactive antigen in a serological diagnosis of leptospirosis. In the present investigation, we confirmed the structural identity of an exocellular mannan isolated from R. glutinis AHU 3479 and tried to use it as an immunoreactive antigen in a serological diagnosis of leptospirosis. From its chemical analysis and (1)H- and (13)C-labeled nuclear magnetic resonance spectrometry, the Rhodotorula mannan was confirmed to consist of the same disaccharide units. Furthermore, such a preparation was shown to immunoreact to various sera from patients suffering with leptospirosis as well as to most rabbit antiserum preparations obtained from immunization with various strains of pathogenic leptospires. Therefore, the Rhodotorula mannan preparation is useful as an immunoreactive antigen in the serological diagnosis for leptospirosis.
此前，有报道称粘红酵母会产生大量胞外甘露聚糖，其重复单元为→3)-D-甘露糖-(1→4)-D-甘露糖-(1→。最近，我们发现双曲钩端螺旋体帕托克血清型帕托克I菌株的抗原性多糖具有相同的重复单元，并且能与针对其他钩端螺旋体延伸菌株产生的抗血清发生交叉反应（K. 松尾、E. 矶贝和Y. 荒木，《碳水化合物研究》，即将发表）。这种结构上的一致性以及生产和分离抗原的困难促使我们确认粘红酵母甘露聚糖作为钩端螺旋体病血清学诊断中一种免疫反应性抗原的实用性。在本研究中，我们确认了从粘红酵母AHU 3479分离出的胞外甘露聚糖的结构一致性，并试图将其用作钩端螺旋体病血清学诊断中的免疫反应性抗原。通过化学分析以及¹H和¹³C标记的核磁共振光谱法，确认粘红酵母甘露聚糖由相同的二糖单元组成。此外，这种制剂被证明能与钩端螺旋体病患者的各种血清以及从用各种致病性钩端螺旋体菌株免疫获得的大多数兔抗血清制剂发生免疫反应。因此，粘红酵母甘露聚糖制剂作为钩端螺旋体病血清学诊断中的免疫反应性抗原是有用的。
