Wilder T D, Barbaree J M, Macklin K S, Norton R A
Department of Biological Sciences, Auburn University, AL 36849-5416, USA.
Avian Dis. 2001 Jul-Sep;45(3):659-62.
A gangrenous dermatitis model was developed in broiler chickens, in which birds previously vaccinated at 14 days of age with a bursal disease virus vaccine were challenged at 4 wk of age with various bacterial combinations with the combination of subcutaneous and intramuscular injection. Gangrenous dermatitis lesions were not produced in birds injected with one of the Staphylococcus aureus isolates, either alone or in combination with various Clostridium septicum isolates. Other S. aureus isolates produced significant levels of gangrenous dermatitis either alone or in combination with the same C. septicum isolates. These same C. septicum isolates when given alone did not produce gangrenous lesions. Data from this experiment show the highest level of mortality occurred in birds challenged with a mixture of C. septicum and S. aureus isolates, whereas lower or no mortality was associated with the same isolates given separately. The data clearly demonstrate that the pathogenicity of isolates responsible for gangrenous dermatitis varies widely, indicating that the frequency and severity of lesion production, as well as the occurrence of mortality, are largely dependent upon the specific isolate or isolates with which the birds are challenged.
在肉鸡中建立了坏疽性皮炎模型，其中在14日龄时用法氏囊病病毒疫苗预先接种的鸡，在4周龄时通过皮下和肌肉注射各种细菌组合进行攻毒。单独注射金黄色葡萄球菌分离株之一或与各种败血梭菌分离株联合注射时，均未在鸡中产生坏疽性皮炎病变。其他金黄色葡萄球菌分离株单独或与相同的败血梭菌分离株联合使用时，均产生了显著水平的坏疽性皮炎。这些相同的败血梭菌分离株单独使用时不会产生坏疽病变。该实验数据表明，用败血梭菌和金黄色葡萄球菌分离株混合物攻毒的鸡死亡率最高，而单独给予相同分离株时死亡率较低或无死亡。数据清楚地表明，导致坏疽性皮炎的分离株致病性差异很大，这表明病变产生的频率和严重程度以及死亡率的发生在很大程度上取决于攻毒鸡所接触的特定分离株。
