Kim T J, Tripathy D N
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61802, USA.
Avian Dis. 2001 Jul-Sep;45(3):663-9.
Integration of reticuloendotheliosis virus (REV) into the genome of fowl poxvirus (FPV) has been reported recently. With a view to determine whether this event had occurred in the past, we screened by polymerase chain reaction (PCR) for the presence of REV provirus in the DNAs of nine avian poxviruses, some of which had been lyophilized 50 yr ago. For REV, 5' long terminal repeat (LTR) and REV envelope sequences were amplified, whereas for FPV, the major envelope antigen gene and the region flanking REV sequences were amplified. In six of seven FPV strains examined, the specific PCR amplicons were obtained for both REV provirus and FPV sequences. One isolate in which presence of REV 5' LTR and envelope was not detected by PCR, a LTR remnant was detected by Southern hybridization. Interestingly, no REV sequence was detected in either canary poxvirus or pigeon poxvirus genome. These observations indicate that REV integration in the FPV genome is not a recent phenomenon but probably occurred prior to 1949.
最近有报道称，网状内皮组织增生症病毒（REV）整合到了禽痘病毒（FPV）的基因组中。为了确定这种情况过去是否发生过，我们通过聚合酶链反应（PCR）对9种禽痘病毒的DNA进行筛查，寻找REV前病毒的存在，其中一些病毒在50年前就已冻干。对于REV，扩增5'长末端重复序列（LTR）和REV包膜序列，而对于FPV，扩增主要包膜抗原基因和REV序列侧翼区域。在检测的7株FPV毒株中，有6株获得了REV前病毒和FPV序列的特异性PCR扩增产物。有一个分离株，PCR未检测到REV 5' LTR和包膜的存在，但通过Southern杂交检测到了LTR残余物。有趣的是，在金丝雀痘病毒或鸽痘病毒基因组中均未检测到REV序列。这些观察结果表明，REV整合到FPV基因组中并非近期现象，可能发生在1949年之前。
