Biswas Sanchay K, Jana Chandrakanta, Chand Karam, Rehman Waseem, Mondal Bimalendu
Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital 263 138, Uttarakhand, India.
Vet Ital. 2011 Apr-Jun;47(2):147-53.
Fowl poxvirus (FPV) infection was observed in unvaccinated backyard chickens. A total of 15 birds were affected in a flock of 37. Pock lesions were observed on the comb, eyelids, beak and wattles. The birds appeared sick with roughened feathers and stunted growth. No mortality was recorded. DNA was isolated from scabs and polymerase chain reaction (PCR) was performed to amplify the 4b core protein gene of FPV, the envelope (env) gene of reticuloendotheliosis virus (REV) and the region of FPV flanking REV 5´ long terminal repeat (LTR). Correct-size PCR products of 578 bp, 807 bp and 370 bp, respectively, were observed in agarose gel electrophoresis. Sequence analysis of these products suggests that the virus was an FPV with a genome containing an integrated near full-length REV provirus. Given the fact that REV has been associated with immunosuppression, its presence in the genome of FPV appears to play an important role in the pathogenesis of fowl pox and presumably prolongs persistence of FPV in bird populations. In the present case, fowl pox has been observed to have persisted for about three years in fowl that were reared in backyard systems in villages.
在未接种疫苗的后院鸡群中观察到禽痘病毒（FPV）感染。一个37只鸡的鸡群中共有15只鸡受到影响。在鸡冠、眼睑、喙和肉垂上观察到痘疹病变。这些鸡看起来患病，羽毛粗糙且生长发育迟缓。未记录到死亡情况。从痂皮中分离出DNA，并进行聚合酶链反应（PCR）以扩增FPV的4b核心蛋白基因、网状内皮组织增生症病毒（REV）的包膜（env）基因以及REV 5´长末端重复序列（LTR）侧翼的FPV区域。在琼脂糖凝胶电泳中分别观察到大小正确的578 bp、807 bp和370 bp的PCR产物。对这些产物的序列分析表明，该病毒是一种FPV，其基因组包含一个整合的近全长REV前病毒。鉴于REV与免疫抑制有关，它在FPV基因组中的存在似乎在禽痘的发病机制中起重要作用，并且可能延长FPV在鸡群中的持续存在时间。在本病例中，观察到禽痘在村庄后院养殖系统中的鸡群中持续存在了约三年。
