Maliwal B P, Gryczynski Z, Lakowicz J R
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 21201, USA.
Anal Chem. 2001 Sep 1;73(17):4277-85. doi: 10.1021/ac0101050.
We describe a new approach to making luminophores that display long emission wavelengths, long decay times, and high quantum yields. These luminophores are covalently linked pairs with a long-lifetime resonance-energy-transfer donor and a long-wavelength acceptor. The donor was a ruthenium (Ru) metal-ligand complex. The acceptor was the Texas Red. The donor and acceptor were covalently linked by polyproline spacers. The long-lifetime donor results in a long-lived component in the acceptor decay, which is due to RET. Importantly, the quantum yield of the luminophores approaches that of the higher quantum yield acceptor, rather than the lower quantum yield typical of metal-ligand complexes. The emission maxima and decay time of such tandem luminophores can be readily adjusted by selection of the donor, acceptor, and distance between them. Luminophores with these useful spectral properties can also be donor-acceptor pairs brought into close proximity by some biochemical association reaction. Luminophores with long-wavelength emission and long lifetimes can have numerous applications in biophysics, clinical diagnostics, DNA analysis, and drug discovery.
我们描述了一种制备发光体的新方法，这些发光体具有长发射波长、长衰减时间和高量子产率。这些发光体是由具有长寿命共振能量转移供体和长波长受体的共价连接对组成。供体是钌（Ru）金属-配体配合物。受体是德克萨斯红。供体和受体通过聚脯氨酸间隔基共价连接。长寿命供体导致受体衰减中出现长寿命成分，这是由于共振能量转移所致。重要的是，发光体的量子产率接近较高量子产率受体的量子产率，而不是金属-配体配合物典型的较低量子产率。通过选择供体、受体以及它们之间的距离，可以很容易地调节这种串联发光体的发射最大值和衰减时间。具有这些有用光谱特性的发光体也可以是通过某些生化缔合反应紧密靠近的供体-受体对。具有长波长发射和长寿命的发光体在生物物理学、临床诊断、DNA分析和药物发现等方面有众多应用。
