Carlsson P O, Jansson L
Department of Medical Cell Biology, Uppsala University, Biomedical Centre, Box 571, SE-751 23 Uppsala, Sweden.
World J Surg. 2001 Jul;25(7):835-9. doi: 10.1007/s00268-001-0037-0.
Secretin is a stimulator of both endocrine and exocrine secretions of the pancreas, and we aimed to evaluate its effects on splanchnic blood flow in rats with a microsphere technique. Anesthetized rats were infused with secretin (0.5 or 2.0 micrograms/kg body weight/hr) for 10 minutes. Some animals were normoglycemic, whereas other received a glucose injection 3 minutes before blood flow measurements. Secretin did not affect serum insulin concentrations in normoglycemic animals but consistently led to higher insulin concentrations in the hyperglycemic rats. Total pancreatic blood flow was increased by the highest secretin dose in normoglycemic animals, whereas no effects were seen in the hyperglycemic rats. Administration of glucose caused a pronounced increase in islet and fractional islet blood flow in saline-infused animals. Secretin affected neither islet nor fractional blood flow in normoglycemic or hyperglycemic rats. Glucose administration increased duodenal blood flow in animals infused with saline and both duodenal and colonic blood flow in rats given the lowest dose of secretin. No effects on either colonic or duodenal blood perfusion were seen in animals infused with the highest dose of secretin. Secretin mainly affects blood flow to the whole pancreas and not that of the islets. Furthermore, glucose-induced insulin release can be achieved without a simultaneous increase in islet blood flow; that is these two events may be dissociated from one another.
促胰液素是胰腺内分泌和外分泌的刺激物，我们旨在用微球技术评估其对大鼠内脏血流的影响。给麻醉的大鼠输注促胰液素（0.5或2.0微克/千克体重/小时），持续10分钟。一些动物血糖正常，而其他动物在血流测量前3分钟接受葡萄糖注射。促胰液素对血糖正常的动物血清胰岛素浓度没有影响，但持续导致高血糖大鼠的胰岛素浓度升高。促胰液素最高剂量使血糖正常的动物胰腺总血流量增加，而高血糖大鼠未见此效应。给予葡萄糖使输注生理盐水的动物胰岛和胰岛部分血流量显著增加。促胰液素对血糖正常或高血糖大鼠的胰岛或部分血流量均无影响。给予葡萄糖使输注生理盐水的动物十二指肠血流量增加，使给予最低剂量促胰液素的大鼠十二指肠和结肠血流量均增加。给予促胰液素最高剂量的动物，结肠或十二指肠血流灌注均无影响。促胰液素主要影响整个胰腺的血流，而非胰岛的血流。此外，葡萄糖诱导的胰岛素释放可在胰岛血流量不增加的情况下实现；也就是说，这两个事件可能彼此分离。
