Zhu Peixuan, Shelton Daniel R, Karns Jeffrey S, Sundaram Appavu, Li Shuhong, Amstutz Pete, Tang Cha-Mei
Creatv MicroTech, Inc., 11609 Lake Potomac Drive, Potomac, MD 20854, USA.
Biosens Bioelectron. 2005 Oct 15;21(4):678-83. doi: 10.1016/j.bios.2005.01.005.
Escherichia coli O157:H7, the most common serotype of enterohemorrhagic E. coli (EHEC), is responsible for numerous food-borne and water-borne infections worldwide. An integrating waveguide biosensor is described for the detection of water-borne E. coli O157, based on a fluorescent sandwich immunoassay performed inside a glass capillary waveguide. The genomic DNA of captured E. coli O157 cells was extracted and quantitative real-time PCR subsequently performed to assess biosensor-capture efficiency. In vitro microbial growth in capillary waveguide is also documented. The biosensor allows for quantitative detection of as few as 10 cells per capillary (0.075 ml volume) and can be used in conjunction with cell amplification, PCR and microarray technologies to positively identify a pathogen.
肠出血性大肠杆菌（EHEC）最常见的血清型大肠杆菌O157:H7，在全球范围内引发了众多食源性和水源性感染。本文描述了一种集成波导生物传感器，用于检测水源性大肠杆菌O157，该传感器基于在玻璃毛细管波导内进行的荧光夹心免疫测定法。提取捕获的大肠杆菌O157细胞的基因组DNA，随后进行定量实时PCR以评估生物传感器的捕获效率。还记录了毛细管波导中的体外微生物生长情况。该生物传感器能够对每根毛细管（体积为0.075毫升）中低至10个细胞进行定量检测，并且可与细胞扩增、PCR和微阵列技术结合使用，以阳性鉴定病原体。
