Kamakura S, Sasano Y, Homma H, Suzuki O, Kagayama M, Motegi K
Division of Stomatology and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Japan.
Oral Dis. 2001 Jul;7(4):259-65.
Our previous radiographic examinations have indicated that the synthetic octacalcium phosphate (OCP) may provide the core for nucleating multiple osteogenic sites in the experimentally created cranial defect.
The present study was designed to confirm the possibility that the implanted OCP causes the osteoinduction as well as the osteoconduction in the rat cranial defect.
Standardized defects were created in male Wistar rat calvaria, and the OCP granules were implanted into the defect. The sham operated rats were processed in the same way except that nothing was implanted. The rats were fixed at 4 weeks after implantation of OCP or the sham operation. We examined bone formed on the implanted OCP, analyzing serial sections histologically combined with immunohistochemistry for the bone specific protein, osteocalcin.
In the defects treated with OCP, the radiopacity was scattered throughout the defect besides being observed along the defect margin of the parietal bone. Examination of the serial sections showed that some of new bones on the implanted OCP were formed away from the defect margin of the parietal bone with regard to both histological identification and specific molecular marker.
The present study suggested that the implanted OCP can serve as a core for initiating bone formation and cause the osteoinduction as well as the osteoconduction in the defect.
我们之前的影像学检查表明，合成八钙磷酸酯（OCP）可能为在实验性颅骨缺损中形成多个成骨位点提供核心。
本研究旨在证实植入的OCP在大鼠颅骨缺损中引起骨诱导和骨传导的可能性。
在雄性Wistar大鼠颅骨上制造标准化缺损，并将OCP颗粒植入缺损处。假手术大鼠的处理方式相同，但不植入任何东西。在植入OCP或进行假手术后四周将大鼠处死。我们检查了植入OCP上形成的骨，通过组织学连续切片结合骨特异性蛋白骨钙素的免疫组织化学分析。
在用OCP处理的缺损中，除了沿顶骨缺损边缘观察到不透射线外，不透射线还散布在整个缺损处。连续切片检查显示，就组织学鉴定和特异性分子标记而言，植入OCP上的一些新骨是在远离顶骨缺损边缘的地方形成的。
本研究表明，植入的OCP可作为启动骨形成的核心，并在缺损中引起骨诱导和骨传导。
