Igartuburu J M, Pando E, Rodríguez Luis F, Gil-Serrano A
Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, Apartado 40, 11510-Puerto Real, Cádiz, Spain.
J Nat Prod. 2001 Sep;64(9):1174-8. doi: 10.1021/np000363c.
The structure of a hemicellulose B fraction (B-1) isolated from grape skins (Vitis vinifera) of the Palomino variety has been studied by methylation analysis, (1)H NMR and (13)C NMR spectroscopy, and partial acid hydrolysis. Hemicellulose B-1 appeared to be homogeneous by gel filtration with a weight-average molecular weight of 22 600. This polysaccharide is a linear xyloglucan chain composed of xylopyranosyl and glucopyranosyl residues linked by beta-(1-->4) glycosidic bonds. Attached to this backbone, 4-O-methyl-D-glucuronopyranosyl acid, D-glucopyranosyl, and L-fucopyranosyl residues occur at position 2 in a ratio of one residue for every five units of xylose in the main chain, with D-xylopyranosyl residues attached at position 6 of glucose units in a ratio of one residue for every two glucose-derived moieties in the main chain.
通过甲基化分析、¹H NMR和¹³C NMR光谱以及部分酸水解，对从帕洛米诺品种葡萄皮（葡萄属）中分离出的半纤维素B级分（B-1）的结构进行了研究。通过凝胶过滤，半纤维素B-1似乎是均匀的，重均分子量为22600。这种多糖是由吡喃木糖基和吡喃葡萄糖基残基通过β-(1→4)糖苷键连接而成的线性木葡聚糖链。连接在该主链上，4-O-甲基-D-葡糖醛酸吡喃糖基酸、D-吡喃葡萄糖基和L-岩藻糖吡喃糖基残基在第2位以每五个主链木糖单元一个残基的比例出现，D-吡喃木糖基残基以每两个主链葡萄糖衍生部分一个残基的比例连接在葡萄糖单元的第6位。
