Li Yan, Wang Yan-Liang, Li Li, Liu Liu, Lu Yu-Xin, Cheng Xiao-Chen, Zhang Qing-Lin
Beijing Institute of Radiation Medicine, Beijing, China.
J Asian Nat Prod Res. 2009 Nov;11(11):951-7. doi: 10.1080/10286020903305896.
Three polysaccharides were isolated from the roots of Urtica fissa by extraction, ultrafiltration, anion-exchange, and gel-filtration chromatography. The structures were characterized using acetylation, methylation, and spectral methods (GCMS, NMR). All three polysaccharides are mainly composed of D-arabinofuranosyl, D-galactopyranosyl, D-glucopyranosyl residues with different structural characteristics. Polysaccharide A of MW 5.2 x 10(3) contained a linear chain of 1-linked beta-D-glucopyranosyl, 1,6-linked beta-D-glucopyranosyl, 1,6-linked alpha-galactopyranosyl, and 1,5-linked beta-arabinofuranosyl moieties. Polysaccharide B of MW 7.7 x 10(4) possessed a chain consisting of 1,5-linked alpha-D-arabinofuranosyl, 1,3-linked beta-D-mannopyranosyl, 1,6-linked beta-D-glucopyranosyl, and 1,6-linked alpha-D-galactopyranosyl residues, but 4-O of alpha-D-galactopyranosyl residues were branched by terminal beta-D-glucopyranosyl residues. Polysaccharide C of MW 5.3 x 10(4) composed of a chain of 1,5-linked alpha-D-arabinofuranosyl, 1,4-linked beta-D-galactopyranosyl, 1,5-linked beta-D-xylopyranosyl, 1,4-linked beta-D-mannopyranosyl, 1-linked beta-D-glucopyranosyl residues, and the terminal beta-D-glucopyranosyl residues are attached to 3-O positions of 1,6-linked alpha-D-glucopyranosyl residues.
通过提取、超滤、阴离子交换和凝胶过滤色谱法从裂叶荨麻根中分离出三种多糖。采用乙酰化、甲基化和光谱方法（气相色谱 - 质谱联用、核磁共振）对其结构进行表征。所有三种多糖主要由具有不同结构特征的D - 阿拉伯呋喃糖基、D - 吡喃半乳糖基、D - 吡喃葡萄糖基残基组成。分子量为5.2×10³的多糖A含有由1 - 连接的β - D - 吡喃葡萄糖基、1,6 - 连接的β - D - 吡喃葡萄糖基、1,6 - 连接的α - D - 吡喃半乳糖基和1,5 - 连接的β - D - 阿拉伯呋喃糖基部分组成的线性链。分子量为7.7×10⁴的多糖B具有由1,5 - 连接的α - D - 阿拉伯呋喃糖基、1,3 - 连接的β - D - 甘露吡喃糖基、1,6 - 连接的β - D - 吡喃葡萄糖基和1,6 - 连接的α - D - 吡喃半乳糖基残基组成的链，但α - D - 吡喃半乳糖基残基的4 - O位被末端β - D - 吡喃葡萄糖基残基分支。分子量为5.3×10⁴的多糖C由1,5 - 连接的α - D - 阿拉伯呋喃糖基、1,4 - 连接的β - D - 吡喃半乳糖基、1,5 - 连接的β - D - 木吡喃糖基、1,4 - 连接的β - D - 甘露吡喃糖基、1 - 连接的β - D - 吡喃葡萄糖基残基组成的链，并且末端β - D - 吡喃葡萄糖基残基连接到1,6 - 连接的α - D - 吡喃葡萄糖基残基的3 - O位。
