Blackall P J, Pahoff J L, Marks D, Fegan N, Morrow C J
Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, New South Wales.
Aust Vet J. 1995 Apr;72(4):135-8. doi: 10.1111/j.1751-0813.1995.tb15033.x.
Biochemical profiles, restriction endonuclease analysis (REA) and ribotyping were used to investigate Pasteurella multocida isolates from outbreaks of fowl cholera on 7 turkey farms in New South Wales. While only a single isolate was available from 5 of the farms, multiple isolates, 4 and 12 respectively, were available from the other 2 farms. The available field evidence suggested that 8 outbreaks had occurred with one farm suffering 2 outbreaks. The isolates obtained were all confirmed as Pasteurella multocida. Biochemical profiles allocated the isolates to 4 groups, 3 being variants of P multocida subsp multocida and the fourth being P multocida subsp septica. REA performed with HpaII established 7 groups. Ribotyping using the HpaII digests probed with the 16S rRNA operon of Haemophilus paragallinarum recognised the same 7 groups as REA. Unlike the biochemical profiles, both REA and ribotyping provided a fine subdivision that identified outbreaks as either related or unrelated. The REA and ribotyping patterns as well as biochemical profiles were stable for all isolates from the outbreaks in which multiple isolates were obtained from either the same bird or from different birds. REA and ribotyping were found to be superior to biotyping methods for the investigation of fowl cholera outbreaks.
利用生化特征分析、限制性内切酶分析（REA）和核糖体分型技术，对新南威尔士州7个火鸡养殖场禽霍乱疫情中的多杀性巴氏杆菌分离株进行了研究。虽然5个养殖场仅获得了单个分离株，但另外2个养殖场分别获得了多个分离株，数量为4株和12株。现有的现场证据表明，共发生了8起疫情，其中一个养殖场遭受了2次疫情。所获得的分离株均被确认为多杀性巴氏杆菌。生化特征分析将这些分离株分为4组，其中3组为多杀性巴氏杆菌多杀亚种的变种，第4组为多杀性巴氏杆菌败血亚种。用HpaII进行的REA分析确定了7个组。使用副鸡嗜血杆菌16S rRNA操纵子探针的HpaII酶切产物进行核糖体分型，识别出与REA相同的7个组。与生化特征分析不同，REA和核糖体分型都提供了精细的细分，能够确定疫情之间是否相关。对于从同一禽类或不同禽类中获得多个分离株的疫情中的所有分离株，REA和核糖体分型模式以及生化特征都是稳定的。研究发现，在调查禽霍乱疫情方面，REA和核糖体分型优于生物分型方法。
