核心3上携带的Sd(a)抗原样结构是正常人降结肠粘蛋白聚糖的显著特征。
Sd(a)-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon.
作者信息
Capon C, Maes E, Michalski J C, Leffler H, Kim Y S
机构信息
Laboratoire de Glycobiologie structurale et fonctionnelle, Unité Mixte du Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq Cedex, France.
出版信息
Biochem J. 2001 Sep 15;358(Pt 3):657-64. doi: 10.1042/bj3580657.
Abstract
This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. The saccharides were mainly based on core 3 (GlcNAcbeta1-3GalNAc). Among the terminal saccharide determinants Sd(a)/Cad-antigen-like structures were prominent, and Lewis x, sialyl Lewis x and sulphated Lewis x were found as minor components, whereas blood group H and A antigenic determinants were absent. The saccharides were markedly different from those of mucins from colon cancers or colon cancer cell lines analysed so far, in which cores 1 and 2 are prominent features, and in which various other terminal determinants have been found, but not Sd(a)/Cad.
摘要
本文描述了对尸检时新鲜获取的正常人降结肠粘蛋白聚糖进行核磁共振（NMR）、质谱（MS）结构表征及降解研究。糖类主要基于核心3（β1-3连接的N-乙酰葡糖胺和N-乙酰半乳糖胺）。在末端糖类决定簇中，Sd(a)/Cad抗原样结构显著，而Lewis x、唾液酸化Lewis x和硫酸化Lewis x为次要成分，血型H和A抗原决定簇不存在。这些糖类与迄今为止分析的结肠癌或结肠癌细胞系粘蛋白中的糖类明显不同，后者的主要特征是核心1和核心2，且发现了各种其他末端决定簇，但没有Sd(a)/Cad。
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