He Z, Honeycutt C W
USDA Agricultural Research Service, New England Plant, Soil, and Water Lab., Univ. of Maine, Orono 04469, USA.
J Environ Qual. 2001 Sep-Oct;30(5):1685-92. doi: 10.2134/jeq2001.3051685x.
Information on the forms of P present in animal manure may improve our ability to manage manure P. In most investigations of manure P composition, only inorganic and total P are determined, and the difference between them is assigned as organic P. In this study, we explored the possibility of identifying and quantifying more specific organic P forms in animal manure with orthophosphate-releasing enzymes. Pig (Sus scrofa) manure and cattle (Bos taurus) manure were first sequentially fractionated into water-soluble P, NaHCO3-soluble P, NaOH-soluble P, HCl-soluble P, and residual P. The fractions were separately incubated with wheat phytase, alkaline phosphatase, nuclease P1, nucleotide pyrophosphatase, or their combinations. The released orthophosphate was determined by a molybdate blue method. Part of the organic P in those fractions could be identified by the enzymatic treatments as phytate (i.e., 39% for pig manure and 17% for cattle manure in water-soluble organic P), simple phosphomonoesters (i.e., 43% for pig manure and 15% for cattle manure in NaOH-soluble organic P), nucleotide-like phosphodiesters (2-12%), and nucleotide pyrophosphate (0-4%). Our data indicate that the enzymatic treatment is an effective approach to identify and quantify the organic P forms present in animal manures.
有关动物粪便中磷（P）存在形式的信息，可能会提高我们管理粪便磷的能力。在大多数粪便磷成分研究中，仅测定无机磷和总磷，两者之差被认定为有机磷。在本研究中，我们探讨了用释放正磷酸盐的酶来鉴定和定量动物粪便中更具体有机磷形式的可能性。猪（Sus scrofa）粪和牛（Bos taurus）粪首先依次分级为水溶性磷、碳酸氢钠溶性磷、氢氧化钠溶性磷、盐酸溶性磷和残留磷。这些级分分别与小麦植酸酶、碱性磷酸酶、核酸酶P1、核苷酸焦磷酸酶或它们的组合一起孵育。通过钼酸蓝法测定释放的正磷酸盐。这些级分中的部分有机磷可通过酶处理鉴定为植酸盐（即水溶性有机磷中猪粪为39%，牛粪为17%）、简单磷酸单酯（即氢氧化钠溶性有机磷中猪粪为43%，牛粪为15%）、类核苷酸磷酸二酯（2 - 12%）和核苷酸焦磷酸（0 - 4%）。我们的数据表明，酶处理是鉴定和定量动物粪便中有机磷形式的有效方法。
