Kanouchi H, Tachibana H, Oka T, Yamada K
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Cell Mol Life Sci. 2001 Aug;58(9):1340-3. doi: 10.1007/PL00000945.
Perchloric acid-soluble protein (PSP) may play an important role in the regulation of cellular physiological functions because it has been highly conserved throughout evolution; however, this role has not been well elucidated. In previous reports, we suggested that PSP regulates cell proliferation. In this study, we examined the effect of PSP expression on proliferation of the normal rat kidney cell line NRK-52E, the rat hepatocyte cell line RLN-10, and the rat hepatoma cell line dRLh-84. Cells transfected with pcDNA-sense-PSP (pcDNA-S-PSP) over-expressed PSP mRNA and protein, and cell proliferation of the transfected cells was suppressed compared with that of cells transfected with pcDNA-empty (pcDNA-E). Cell viability of pcDNA-S-PSP-transfected cells was similar to that of pcDNA-E-transfected cells. Thus, over-expression of PSP suppresses cell proliferation without any influence on cell viability. These findings are the first to report an inhibitory activity of PSP on cell proliferation.
高氯酸可溶性蛋白（PSP）可能在细胞生理功能调节中发挥重要作用，因为它在整个进化过程中高度保守；然而，这一作用尚未得到充分阐明。在之前的报告中，我们认为PSP调节细胞增殖。在本研究中，我们检测了PSP表达对正常大鼠肾细胞系NRK-52E、大鼠肝细胞系RLN-10和大鼠肝癌细胞系dRLh-84增殖的影响。用pcDNA-正义-PSP（pcDNA-S-PSP）转染的细胞中PSP mRNA和蛋白过表达，与用pcDNA空载体（pcDNA-E）转染的细胞相比，转染细胞的增殖受到抑制。pcDNA-S-PSP转染细胞的细胞活力与pcDNA-E转染细胞相似。因此，PSP的过表达抑制细胞增殖，而对细胞活力无任何影响。这些发现首次报道了PSP对细胞增殖的抑制活性。
