Hoff G E, Frasch C E
Infect Immun. 1979 Sep;25(3):849-56. doi: 10.1128/iai.25.3.849-856.1979.
This study shows that the capsular polysaccharide, protein, and lipopolysaccharide antigens from the outer membrane of Neisseria meningitidis group B serotype 2 may be identified by crossed immunoelectrophoresis. By using this technique, seven precipitates were resolved when outer membrane preparations were reacted against goat anti-whole cell group B type 2 antiserum. Most of these precipitates were identified by comparison with purified reference preparations. Different outer membrane preparations, reflecting different growth conditions, varied in their compositions of lipopolysaccharide, protein, and polysaccharide. Detergent treatment altered the protein and lipopolysaccharide precipitation patterns. In the presence of detergent, the lipopolysaccharide did not precipitate, and the electrophoretic migration of the protein antigens decreased. Crossed immunoelectrophoresis is a useful qualitative method for analysis of the antigenic components of the meningococcal outer membrane. The crossed immunoelectrophoresis with intermediate gel technique is presently being used to measure the human immune response to the different cell surface components.
本研究表明，B群2型脑膜炎奈瑟菌外膜的荚膜多糖、蛋白质和脂多糖抗原可通过交叉免疫电泳进行鉴定。运用该技术，当外膜制剂与山羊抗B群2型全细胞抗血清反应时，可分辨出七种沉淀物。通过与纯化的参考制剂比较，鉴定出了其中的大多数沉淀物。反映不同生长条件的不同外膜制剂，其脂多糖、蛋白质和多糖的组成有所不同。去污剂处理改变了蛋白质和脂多糖的沉淀模式。在去污剂存在的情况下，脂多糖不沉淀，蛋白质抗原的电泳迁移率降低。交叉免疫电泳是分析脑膜炎球菌外膜抗原成分的一种有用的定性方法。目前正在使用带有中间凝胶技术的交叉免疫电泳来测量人体对不同细胞表面成分的免疫反应。
