脑膜炎奈瑟菌体外生长期间以细胞壁泡形式释放内毒素。
Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis.
作者信息
Devoe I W, Gilchrist J E
出版信息
J Exp Med. 1973 Nov 1;138(5):1156-67. doi: 10.1084/jem.138.5.1156.
Abstract
Multiple cell wall blebs were observed on the surface of three strains of N. meningitidis taken from log phase cultures. The blebs originated as evaginations of the outer layer of the cell wall. Bleb production was noted on both defined or complex media either as broth or a solid medium. The addition of 10% normal bovine serum to the various media did not affect the production and release of these surface blebs. However, as broth cultures progressed into the stationary phase of growth, the blebs disappeared from the surface of the cells. Blebs were present in substantial quantities in culture supernatant fluids and on cell surfaces and were readily isolated by ultracentrifugation. Analysis for 2-keto-3-deoxyoctonate in cultures revealed that 18% of the total endotoxin of log phase cultures was present in blebs from the cell wall.
摘要
在取自对数期培养物的三株脑膜炎奈瑟菌表面观察到多个细胞壁泡。这些泡起源于细胞壁外层的外翻。在限定培养基或复合培养基上，无论是肉汤还是固体培养基，均观察到泡的产生。向各种培养基中添加10%正常牛血清并不影响这些表面泡的产生和释放。然而，随着肉汤培养物进入生长稳定期，泡从细胞表面消失。泡大量存在于培养上清液和细胞表面，通过超速离心很容易分离出来。对培养物中的2-酮-3-脱氧辛酸进行分析发现，对数期培养物中18%的总内毒素存在于细胞壁泡中。
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