Striz I, Slavcev A, Kalanin J, Jaresová M, Rennard S I
Department of Immunology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Inflammation. 2001 Aug;25(4):241-6. doi: 10.1023/a:1010975804179.
Interactions of macrophages with epithelium represent one of the pathways involved in regulating local immune mechanisms. We studied the effect of cell-cell contact with an epithelial monolayer on the phenotype of macrophages. Human monocytes and THP-1 macrophages were co-cultured with monolayers of human bronchial epithelial cells (HBECs), the alveolar type II-like cell line A549, renal adenocarcinoma epithelial cells (RA), and the lung fibroblast strain HFL-1. The expression of CD11b, CD14, CD54, and HLA-DR was measured by immunocytochemistry and flow cytometry and showed epithelial cell induction of CD54 and HLA-DR in monocytes and of all antigens in THP-1 cells. Co-culture with fibroblasts did not change the phenotype of macrophages. Separation by a filter insert inhibited most of the effects. Culture supernatants did not induce prominent phenotypic changes. Cell-cell contacts with epithelium appear to be of importance in regulating the phenotype of macrophages.
巨噬细胞与上皮细胞的相互作用是调节局部免疫机制的途径之一。我们研究了与上皮单层细胞进行细胞间接触对巨噬细胞表型的影响。将人单核细胞和THP-1巨噬细胞与人支气管上皮细胞（HBECs）单层、肺泡II型样细胞系A549、肾腺癌上皮细胞（RA）以及肺成纤维细胞系HFL-1共同培养。通过免疫细胞化学和流式细胞术检测CD11b、CD14、CD54和HLA-DR的表达，结果显示上皮细胞可诱导单核细胞中CD54和HLA-DR的表达，以及THP-1细胞中所有抗原的表达。与成纤维细胞共同培养未改变巨噬细胞的表型。用滤膜插入物分隔可抑制大部分效应。培养上清液未诱导明显的表型变化。与上皮细胞的细胞间接触在调节巨噬细胞表型方面似乎很重要。
