Hirooka H, de Koning D J, Harlizius B, van Arendonk J A, Rattink A P, Groenen M A, Brascamp E W, Bovenhuis H
Faculty of Economics, Ryukoku University, Kyoto, Japan.
J Anim Sci. 2001 Sep;79(9):2320-6. doi: 10.2527/2001.7992320x.
A whole-genome scan was conducted using 132 microsatellite markers to identify chromosomal regions that have an effect on teat number. For this purpose, an experimental cross between Chinese Meishan pigs and five commercial Dutch pig lines was used. Linkage analyses were performed using interval mapping by regression under line cross models including a test for imprinting effects. The whole-genome scan revealed highly significant evidence for three quantitative trait loci (QTL) affecting teat number, of which two were imprinted. Paternally expressed (i.e., maternally imprinted) QTL were found on chromosomes 2 and 12. A Mendelian expressed QTL was found on chromosome 10. The estimated additive effects showed that, for the QTL on chromosomes 10 and 12, the Meishan allele had a positive effect on teat number, but, for the QTL on chromosome 2, the Meishan allele had a negative effect on teat number. This study shows that imprinting may play an important role in the expression of teat number.
利用132个微卫星标记进行了全基因组扫描，以确定影响乳头数目的染色体区域。为此，采用了中国梅山猪与五个荷兰商业猪系之间的实验杂交。使用包括印记效应检验在内的品系杂交模型，通过回归区间作图进行连锁分析。全基因组扫描揭示了影响乳头数目的三个数量性状位点（QTL）的高度显著证据，其中两个是印记的。在第2号和第12号染色体上发现了父本表达（即母本印记）的QTL。在第10号染色体上发现了孟德尔表达的QTL。估计的加性效应表明，对于第10号和第12号染色体上的QTL，梅山等位基因对乳头数目有正向影响，但对于第2号染色体上的QTL，梅山等位基因对乳头数目有负向影响。本研究表明，印记可能在乳头数目的表达中起重要作用。
